- MA5-14918 - Invitrogen Antibodies SYK antibody

Submitted references LILRB2-mediated TREM2 signaling inhibition suppresses microglia functions.

Zhao P, Xu Y, Jiang LL, Fan X, Ku Z, Li L, Liu X, Deng M, Arase H, Zhu JJ, Huang TY, Zhao Y, Zhang C, Xu H, Tong Q, Zhang N, An Z
Molecular neurodegeneration 2022 Jun 18;17(1):44

PLCγ2 regulates TREM2 signalling and integrin-mediated adhesion and migration of human iPSC-derived macrophages.

Obst J, Hall-Roberts HL, Smith TB, Kreuzer M, Magno L, Di Daniel E, Davis JB, Mead E
Scientific reports 2021 Oct 6;11(1):19842

Molecular basis of carrageenan-induced cytokines production in macrophages.

Lopes AH, Silva RL, Fonseca MD, Gomes FI, Maganin AG, Ribeiro LS, Marques LMM, Cunha FQ, Alves-Filho JC, Zamboni DS, Lopes NP, Franklin BS, Gombault A, Ramalho FS, Quesniaux VFJ, Couillin I, Ryffel B, Cunha TM
Cell communication and signaling : CCS 2020 Sep 7;18(1):141

TREM2 Alzheimer's variant R47H causes similar transcriptional dysregulation to knockout, yet only subtle functional phenotypes in human iPSC-derived macrophages.

Hall-Roberts H, Agarwal D, Obst J, Smith TB, Monzón-Sandoval J, Di Daniel E, Webber C, James WS, Mead E, Davis JB, Cowley SA
Alzheimer's research & therapy 2020 Nov 16;12(1):151

Diabetic phenotype in mouse and humans reduces the number of microglia around β-amyloid plaques.

Natunen T, Martiskainen H, Marttinen M, Gabbouj S, Koivisto H, Kemppainen S, Kaipainen S, Takalo M, Svobodová H, Leppänen L, Kemiläinen B, Ryhänen S, Kuulasmaa T, Rahunen E, Juutinen S, Mäkinen P, Miettinen P, Rauramaa T, Pihlajamäki J, Haapasalo A, Leinonen V, Tanila H, Hiltunen M
Molecular neurodegeneration 2020 Nov 10;15(1):66

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-Syk pTyr525/526 in extracts from Ramos cells, untreated or treated with anti-IgM, using Phospho-Syk pTyr525/526 monoclonal antibody (Product # MA5-14918) (upper) or a Syk polyclonal antibody (lower).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 Role of TRIF/Syk/reactive oxygen species on Cg-induced Il1b mRNA. Peritoneal macrophages were stimulated with Cg (300 mug/ml) or medium. a ROS production were quantified in supernatant by the fluorescent probe assay (H2DCFDA) after indicated times. b, c The supernatant were collect after pre-treated cells with N-acetylcysteine (NAC - 3 mM) to quantify Il1b mRNA gene expression by qPCR and IL-1beta production by Elisa after Cg (6 h). d Quantification of ROS production by (H2DCFDA probe) in cells from naive WT vs deficient for Trif -/- after Cg (3 h). e, f Pre-treated cells with selective inhibitor of Syk (iSyk 1, 3 muM) and stimulated with Cg (6 h) to quantify IL-1beta by ELISA and Il1b mRNA gene expression induced by Cg (3 h), medium or (iSyk 3 muM). g Quantification of ROS production (H2DCFDA probe) stimulated with Cg and iSyk (3 uM) after 3 h. h Western blotting analysis of (pSyk) expression compared cells from WT vs Trif -/- . Data are represent the mean +- SD of four independent experiments compared Control vs WT (Cg) or WT vs Knockout/Treatments groups to determine the level of statistical significance (* p < 0.05; ** p < 0.01; and *** p < 0.001; ns, not significant)

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 PLCgamma2 regulates TREM2-mediated signalling in iPSC- derived macrophages. ( A ) Representative Western blots showing phospho-SYK after TREM2 ligation using AF1828 in Parent and PLCgamma2 Ko cells. ( B ) Quantification of SYK phosphorylation after TREM2 ligation shows no effect of PLCgamma2 deficiency, n = 4. Full immunoblot images are presented in Supplementary Fig. 9. ( C ) pSYK HTRF assay confirms similar phosphorylation levels upstream of PLCgamma2 upon TREM2 stimulation in Parent and PLCgamma2 Ko cells, n = 3. ( D ) Ca 2+ flux induced by TREM2 ligation is prevented in PLCgamma2 Ko cells. n = 3 ( E ) PLCgamma2 deficiency abolishes TREM2-induced IP1 production as determined by HTRF assay. n = 3 ( F ) Representative Western blots and ( G ) quantification of ERK1/2 phosphorylation shows no major difference between Parent and PLCgamma2 Ko cells. Full immunoblot images are presented in Supplementary Fig. 10. Data shown represent mean +- SEM, two-way ANOVA followed by Bonferroni's multiple comparison test, * p < 0.05, ** p < 0.01, *** p < 0.001.

MA5-14918

Antibody data