RQ5553

antibody from NSJ Bioreagents

Targeting: SYK

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5553

Provider

NSJ Bioreagents

Product name

SYK Antibody / Spleen tyrosine kinase

Antibody type

Polyclonal

Description

This highly specific SYK antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with human, mouse and rat samples.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the SYK antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) placenta and 2) ThP-1 lysate with SYK antibody. Predicted molecular weight ~72 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse 1) thymus, 2) lung and 3) SP20 lysate with SYK antibody. Predicted molecular weight ~72 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of rat thymus lysate with SYK antibody. Predicted molecular weight ~72 kDa.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human ThP-1 cells with SYK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SYK antibody.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human A431 cells with SYK antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= SYK antibody.