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LearnAb109740
antibody from Aladdin Scientific
Targeting: IKBKG
FIP-3, FIP3, Fip3p, IKK-gamma, IP1, IP2, NEMO, ZC2HC9
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- Ab109740 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant IKK Gamma/NEMO Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human IKK Gamma/NEMO Antibody, Recombinant, could be used for WB, ICC, IF and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200Protein FunctionRegulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also considered to be a mediator for TAX activation of NF-kappa-B. Could be implicated in NF-kappa-B-mediated protection from cytokine toxicity (By similarity). Essential for viral activation of IRF3.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 1-419- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant IKK Gamma/NEMO Antibody (Ab109740) - Western Blot All lanes: Recombinant IKK Gamma/NEMO Antibody (Ab109740) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 48 kDa Observed band size: 58, 46, 39 kDa Exposure time: 9.8 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant IKK Gamma/NEMO Antibody (Ab109740) - IF/ICC Immunocytochemistry IKK Gamma/NEMO (green) in HeLa using Recombinant IKK Gamma/NEMO Antibody (Ab109740), and cells were counterstained with DAPI (blue).
