M01802

antibody from Boster Biological Technology

Targeting: ACACA ACAC, ACC, ACC1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M01802

Provider

Boster Biological Technology

Product name

Anti-Acetyl-CoA Carboxylase Monoclonal Antibody

Antibody type

Monoclonal

Description

Rabbit IgG Monoclonal antibody for Q13085 detection. Tested positive for WB IHC in Human Mouse Rat

Reactivity

Human, Mouse, Rat

Host

Rabbit

Antibody clone number

EDI-1

Vial size

100ug

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.

References

Submitted references

Quinacrine attenuates diet-induced obesity by inhibiting adipogenesis via activation of AMPK signaling.

Li X, Zhou J, Hu Z, Han L, Zhao W, Wu T, Li C, Liu J, Kang J, Chen X
European journal of pharmacology 2023 Jan 5;938:175432

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Down-regulation of ACACA suppresses the malignant progression of Prostate Cancer through inhibiting mitochondrial potential.

Zhang H, Liu S, Cai Z, Dong W, Ye J, Cai Z, Han Z, Liang Y, Zhuo Y, Luo Y, Zhu X, Deng Y, Zhang Y, Liu R, Feng Y, Lai J, Zhou R, Tan H, Zhong W
Journal of Cancer 2021;12(1):232-243

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A flavonoid-rich Smilax china L. extract prevents obesity by upregulating the adiponectin-receptor/AMPK signalling pathway and modulating the gut microbiota in mice.

Li X, Yang L, Li J, Lin L, Zheng G
Food & function 2021 Jul 5;12(13):5862-5875

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Trimetazidine improves hepatic lipogenesis and steatosis in non‑alcoholic fatty liver disease via AMPK‑ChREBP pathway.

Zhang Y, Li C, Li X, Wu C, Zhou H, Lu S, Liu X
Molecular medicine reports 2020 Sep;22(3):2174-2182

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ACC1 is overexpressed in liver cancers and contributes to the proliferation of human hepatoma Hep G2 cells and the rat liver cell line BRL 3A.

Ye B, Yin L, Wang Q, Xu C
Molecular medicine reports 2019 May;19(5):3431-3440

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miR-1322 regulates ChREBP expression via binding a 3'-UTR variant (rs1051943).

Zhang Y, Hu SL, Hu D, Jiang JG, Cui GL, Liu XD, Wang DW
Journal of cellular and molecular medicine 2018 Nov;22(11):5322-5332

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of ACACA using anti-ACACA antibody (M01802).ACACA was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACACA Antibody (M01802) overnight at 4°C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image