Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-15025 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Acetyl-CoA Carboxylase Monoclonal Antibody (B.800.8)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- It is not recommended to aliquot this antibody.
- Reactivity
- Human, Mouse, Rat, Hamster
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- B.800.8
- Vial size
- 100 µL
- Concentration
- 210 µg/mL
- Storage
- -20°C
Submitted references Antiobesity and antidiabetic effects of the dairy bacterium Propionibacterium freudenreichii MJ2 in high-fat diet-induced obese mice by modulating lipid metabolism.
Acute hypoxia influences collagen and matrix metalloproteinase expression by human keratoconus cells in vitro.
An M, Park YH, Lim YH
Scientific reports 2021 Jan 28;11(1):2481
Scientific reports 2021 Jan 28;11(1):2481
Acute hypoxia influences collagen and matrix metalloproteinase expression by human keratoconus cells in vitro.
McKay TB, Hjortdal J, Priyadarsini S, Karamichos D
PloS one 2017;12(4):e0176017
PloS one 2017;12(4):e0176017
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HEK-293 (Lane 1), A549 (Lane 2), HeLa (Lane 3), A-431 (Lane 4), PC-3 (Lane 5) and HepG2 (Lane 6). The blot was probed with Acetyl-CoA Carboxylase Monoclonal Antibody (B.800.8) (Product # MA5-15025, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A band at ~270 kDa corresponding to Acetyl-CoA was observed across all the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of AcetylCoA Carboxylase was achieved by transfecting HeLa cells with AcetylCoA Carboxylase specific siRNAs (Silencer® select Product # s882, s883). Western blot analysis (Fig. a) was performed using whole cell extracts from the AcetylCoA Carboxylase knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with AcetylCoA Carboxylase Monoclonal Antibody (B.800.8) (Product # MA5-15025, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to AcetylCoA Carboxylase.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Acetyl-CoA Carboxylase in NIH/3T3 cells using an Acetyl-CoA Carboxylase monoclonal antibody (Product # MA5-15025) (red). DNA is labeled using a fluorescent blue dye.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Acetyl-CoA Carboxylase in paraffin-embedded human lung carcinoma using an Acetyl-CoA Carboxylase monoclonal antibody (Product # MA5-15025).