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- Antibody Data
- Antigen structure
- References [13]
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- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- 15682-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#15682-1-AP, RRID:AB_2146065
- Product name
- MRPL45 antibody
- Antibody type
- Polyclonal
- Description
- MRPL45 antibody (Cat. #15682-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB, ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Identification of TMEM126A as OXA1L-interacting protein reveals cotranslational quality control in mitochondria.
Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease.
Nonstop mRNAs generate a ground state of mitochondrial gene expression noise.
An in vitro system to silence mitochondrial gene expression.
Messenger RNA delivery to mitoribosomes - hints from a bacterial toxin.
Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis.
FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function.
Fidelity of translation initiation is required for coordinated respiratory complex assembly.
RNA modification landscape of the human mitochondrial tRNA(Lys) regulates protein synthesis.
Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.
The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.
Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria.
MPV17L2 is required for ribosome assembly in mitochondria.
Poerschke S, Oeljeklaus S, Cruz-Zaragoza LD, Schenzielorz A, Dahal D, Hillen HS, Das H, Kremer LS, Valpadashi A, Breuer M, Sattmann J, Richter-Dennerlein R, Warscheid B, Dennerlein S, Rehling P
Molecular cell 2024 Jan 18;84(2):345-358.e5
Molecular cell 2024 Jan 18;84(2):345-358.e5
Multi-omics identifies large mitoribosomal subunit instability caused by pathogenic MRPL39 variants as a cause of pediatric onset mitochondrial disease.
Amarasekera SSC, Hock DH, Lake NJ, Calvo SE, Grønborg SW, Krzesinski EI, Amor DJ, Fahey MC, Simons C, Wibrand F, Mootha VK, Lek M, Lunke S, Stark Z, Østergaard E, Christodoulou J, Thorburn DR, Stroud DA, Compton AG
Human molecular genetics 2023 Jul 20;32(15):2441-2454
Human molecular genetics 2023 Jul 20;32(15):2441-2454
Nonstop mRNAs generate a ground state of mitochondrial gene expression noise.
Ng KY, Lutfullahoglu Bal G, Richter U, Safronov O, Paulin L, Dunn CD, Paavilainen VO, Richer J, Newman WG, Taylor RW, Battersby BJ
Science advances 2022 Nov 16;8(46):eabq5234
Science advances 2022 Nov 16;8(46):eabq5234
An in vitro system to silence mitochondrial gene expression.
Cruz-Zaragoza LD, Dennerlein S, Linden A, Yousefi R, Lavdovskaia E, Aich A, Falk RR, Gomkale R, Schöndorf T, Bohnsack MT, Richter-Dennerlein R, Urlaub H, Rehling P
Cell 2021 Nov 11;184(23):5824-5837.e15
Cell 2021 Nov 11;184(23):5824-5837.e15
Messenger RNA delivery to mitoribosomes - hints from a bacterial toxin.
Bruni F, Proctor-Kent Y, Lightowlers RN, Chrzanowska-Lightowlers ZM
The FEBS journal 2021 Jan;288(2):437-451
The FEBS journal 2021 Jan;288(2):437-451
Cardiolipin is required for membrane docking of mitochondrial ribosomes and protein synthesis.
Lee RG, Gao J, Siira SJ, Shearwood AM, Ermer JA, Hofferek V, Mathews JC, Zheng M, Reid GE, Rackham O, Filipovska A
Journal of cell science 2020 Jul 24;133(14)
Journal of cell science 2020 Jul 24;133(14)
FAM92A1 is a BAR domain protein required for mitochondrial ultrastructure and function.
Wang L, Yan Z, Vihinen H, Eriksson O, Wang W, Soliymani R, Lu Y, Xue Y, Jokitalo E, Li J, Zhao H
The Journal of cell biology 2019 Jan 7;218(1):97-111
The Journal of cell biology 2019 Jan 7;218(1):97-111
Fidelity of translation initiation is required for coordinated respiratory complex assembly.
Rudler DL, Hughes LA, Perks KL, Richman TR, Kuznetsova I, Ermer JA, Abudulai LN, Shearwood AJ, Viola HM, Hool LC, Siira SJ, Rackham O, Filipovska A
Science advances 2019 Dec;5(12):eaay2118
Science advances 2019 Dec;5(12):eaay2118
RNA modification landscape of the human mitochondrial tRNA(Lys) regulates protein synthesis.
Richter U, Evans ME, Clark WC, Marttinen P, Shoubridge EA, Suomalainen A, Wredenberg A, Wedell A, Pan T, Battersby BJ
Nature communications 2018 Sep 27;9(1):3966
Nature communications 2018 Sep 27;9(1):3966
Inhibition of mitochondrial translation in fibroblasts from a patient expressing the KARS p.(Pro228Leu) variant and presenting with sensorineural deafness, developmental delay, and lactic acidosis.
Ruzzenente B, Assouline Z, Barcia G, Rio M, Boddaert N, Munnich A, Rötig A, Metodiev MD
Human mutation 2018 Dec;39(12):2047-2059
Human mutation 2018 Dec;39(12):2047-2059
The Pseudouridine Synthase RPUSD4 Is an Essential Component of Mitochondrial RNA Granules.
Zaganelli S, Rebelo-Guiomar P, Maundrell K, Rozanska A, Pierredon S, Powell CA, Jourdain AA, Hulo N, Lightowlers RN, Chrzanowska-Lightowlers ZM, Minczuk M, Martinou JC
The Journal of biological chemistry 2017 Mar 17;292(11):4519-4532
The Journal of biological chemistry 2017 Mar 17;292(11):4519-4532
Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria.
Akman G, Desai R, Bailey LJ, Yasukawa T, Dalla Rosa I, Durigon R, Holmes JB, Moss CF, Mennuni M, Houlden H, Crouch RJ, Hanna MG, Pitceathly RD, Spinazzola A, Holt IJ
Proceedings of the National Academy of Sciences of the United States of America 2016 Jul 26;113(30):E4276-85
Proceedings of the National Academy of Sciences of the United States of America 2016 Jul 26;113(30):E4276-85
MPV17L2 is required for ribosome assembly in mitochondria.
Dalla Rosa I, Durigon R, Pearce SF, Rorbach J, Hirst EM, Vidoni S, Reyes A, Brea-Calvo G, Minczuk M, Woellhaf MW, Herrmann JM, Huynen MA, Holt IJ, Spinazzola A
Nucleic acids research 2014 Jul;42(13):8500-15
Nucleic acids research 2014 Jul;42(13):8500-15
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 15682-1-AP(MRPL45 antibody) at dilution of 1:500
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- IP Result of anti-MRPL45 (IP:15682-1-AP, 3ug; Detection:15682-1-AP 1:500) with HeLa cells lysate 2000ug.
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The MRPL45 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human MRPL45. This antibody recognizes human,mouse,rat antigen. The MRPL45 antibody has been validated for the following applications: ELISA, WB, IHC, IP analysis.
