PA5-64504

antibody from Invitrogen Antibodies

Targeting: FES FPS

Western blot (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-64504

Provider

Invitrogen Antibodies

Product name

Phospho-FES (Tyr713) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Phospho-FES (Tyr713) Polyclonal Antibody detects endogenous levels of FES only when phosphorylated at Tyr713.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

References

Submitted references

Chemical genetics strategy to profile kinase target engagement reveals role of FES in neutrophil phagocytosis.

van der Wel T, Hilhorst R, den Dulk H, van den Hooven T, Prins NM, Wijnakker JAPM, Florea BI, Lenselink EB, van Westen GJP, Ruijtenbeek R, Overkleeft HS, Kaptein A, Barf T, van der Stelt M
Nature communications 2020 Jun 25;11(1):3216

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4 FES S700C can be visualized by fluorescent probe WEL033. a Design of fluorescent probe WEL033 (Cy5-conjugate). b Dose-dependent labeling of full-length FES S700C by WEL033 in HEK293T cell lysate. c Labeling by WEL033 is specific, exclusive for FES S700C and dependent on catalytic lysine 590. Lysates were preincubated with vehicle or 4 and labeled by WEL033 (250 nM). d , e Visualization of FES S700C target engagement by WEL028 and TAE684. Lysate was preincubated with WEL028 or TAE684 and labeled by WEL033 (250 nM). Band intensities were normalized to vehicle-treated control ( n = 3). f , g WEL028 engages recombinantly expressed FES S700C in live cells. Transfected HEK293T cells were treated with WEL028 and labeled proteins were visualized using click chemistry. Band intensities were normalized to highest concentration ( n = 3). h , i WEL028 blocks FES S700C but not FES WT autophosphorylation (visualized by immunoblot using anti-phospho-FES Y713). Band intensities were normalized to vehicle-treated control ( n = 3). Data represent means +- SEM. Statistical analysis: two-tailed t-test: *** P < 0.001. Source data are provided as a Source Data file.