12998-1-AP

antibody from Proteintech Group

Targeting: NEFL CMT1F, CMT2E, NF68, NFL, PPP1R110

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

12998-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#12998-1-AP, RRID:AB_10597388

Product name

NF-L antibody

Antibody type

Polyclonal

Description

NF-L antibody (Cat. #12998-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

Human umbilical cord mesenchymal stem cells alleviate hypoxic-ischemia-induced white-matter injury in neonatal rats by regulating polarization of microglia.

Wang C, Xu QQ, Zhang SJ, Zhu YP
Scientific reports 2026 Mar 3;16(1)

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Rewiring brain structural and functional disconnection with acupuncture in rat model of vascular cognitive impairment and dementia.

Wang L, Zhao JP, Cao Y, Ma SM, Yang JW, Su XT, Huang J, Wang QY, Liu CZ
Biological research 2026 Jan 19;59(1):10

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Periodic Therapeutic Phlebotomy Mitigates Systemic Aging Phenotypes by Promoting Bone Marrow Function.

Cai JR, Zhang J, Ning YX, Zhang J, Xu TC, Liu MC, Wang KX, Chen HS
Aging cell 2026 Feb;25(2):e70400

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An injectable curcumin-loaded hydrogel for neuroprotective treatment promote nerve tissue repair in rat severe spinal cord injury.

Zhang Z, Ma H, Tian R, Li G, Zhao F, Xie L, Chen H
Frontiers in bioengineering and biotechnology 2025;13:1655686

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Aryl Hydrocarbon Receptor-Dependent miRNA-382-5p Mediates the Classical Pyroptosis Induced by Foodborne Benzo(a)pyrene through Targeting IκB in Liver.

Meng Y, Jia Y, He Z, Li J, Yuan L
Journal of agricultural and food chemistry 2025 May 28;73(21):12978-12992

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Conductive MeCbl/PEDOT:PSS/HA hydrogels with electrical stimulation for enhanced peripheral nerve regeneration.

Liu K, Shao J, Han B, Liu J, Yan S, Liu B, Liu Y
Materials today. Bio 2025 Jun;32:101755

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A mass-producible macaque model displays a durable Alzheimer-like cognitive deficit and hallmark amyloid-β/tau/neurofilament light chain pathologies.

He F, Shi WJ, Liu W, Fan JX, He ZG, Zhang YQ, Xiao J, Ruan WW, Gai YK, Zhang HL, Yang BB, Qin Y, Wang H, Li J, Wang JL, Liu S, Shi LP, Chen ZX, Jiang WJ, An N, Xue PJ, Wang ZH, Yang RJ, Tian PY, Chen Z, Xiao L, Yang ZS, Feng KB, Tan WY, Sun ZM, Xu W, Shu H, Wang JZ
Journal of Alzheimer's disease : JAD 2025 Jun;105(4):1432-1446

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Kai-Xin-San ameliorates fluoxetine-resistant depressive-like behaviors by modulating tryptophan-kynurenine metabolic homeostasis in a rodent model.

Yao L, Jing R, Wang C, Wang Y, Li X, Wu X, Wang Y, Liu L, Mu L, Liu P, Hu Y
Journal of ethnopharmacology 2025 Jul 24;351:120011

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Multiparametric grading of glaucoma severity by histopathology can enable post-mortem substratification of disease state.

Xiang C, Raghunathan V, Qiu Y, Mehta M, Demirs JT, Grosskreutz CL, Wilson CW, Prasanna G
Acta neuropathologica communications 2025 Jan 6;13(1):3

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Increased nerve density adversely affects outcome in colorectal cancer and denervation suppresses tumor growth.

Wang H, Huo R, He K, Li W, Gao Y, He W, Yu M, Jiang SH, Xue J
Journal of translational medicine 2025 Jan 23;23(1):112

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Neuronal Differentiation of Human Glioma Cells Induced by Parthenolide Under In Vitro Conditions.

Tang Z, Cao C, Tang W, Ye Y, Chen Z, Shen Y
Biomedicines 2024 Nov 7;12(11)

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Electrochemical Biosensor for Point-of-Care Testing of Low-Abundance Biomarkers of Neurological Diseases.

Huang Z, Zhang L, Dou Y, Liu X, Song S, Jiang H, Fan C
Analytical chemistry 2024 Jun 25;96(25):10332-10340

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Microarray Chip-Based High-Throughput Screening of Neurofilament Light Chain Self-Assembling Peptide for Noninvasive Monitoring of Alzheimer's Disease.

Zhou Y, Cai G, Wang Y, Guo Y, Yang Z, Wang A, Chen Y, Li X, Chen X, Hu Z, Wang Z
ACS nano 2024 Jul 16;18(28):18160-18175

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Bisphenol S impairs mitochondrial function by targeting Myo19/oxidative phosphorylation pathway contributing to axonal and dendritic injury.

Zhang X, Gong H, Zhao Y, Wu Y, Cheng J, Song Y, Wang B, Qin Y, Sun M
Environment international 2024 Apr;186:108643

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C9orf72 poly(PR) mediated neurodegeneration is associated with nucleolar stress.

Cicardi ME, Hallgren JH, Mawrie D, Krishnamurthy K, Markandaiah SS, Nelson AT, Kankate V, Anderson EN, Pasinelli P, Pandey UB, Eischen CM, Trotti D
iScience 2023 Sep 15;26(9):107505

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Early preclinical plasma protein biomarkers of brain trauma are influenced by early seizures and levetiracetam.

Saletti PG, Mowrey WB, Liu W, Li Q, McCullough J, Aniceto R, Lin IH, Eklund M, Casillas-Espinosa PM, Ali I, Santana-Gomez C, Coles L, Shultz SR, Jones N, Staba R, O'Brien TJ, Moshé SL, Agoston DV, Galanopoulou AS, EpiBioS4Rx Study Group
Epilepsia open 2023 Jun;8(2):586-608

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Regulatory T cells alleviate myelin loss and cognitive dysfunction by regulating neuroinflammation and microglial pyroptosis via TLR4/MyD88/NF-κB pathway in LPC-induced demyelination.

Wang Y, Sadike D, Huang B, Li P, Wu Q, Jiang N, Fang Y, Song G, Xu L, Wang W, Xie M
Journal of neuroinflammation 2023 Feb 18;20(1):41

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＜Editors' Choice＞ Supernatant from activated omentum accelerates wound healing in diabetic mice wound model.

Li Y, Hashikawa K, Ebisawa K, Kambe M, Higuchi S, Kamei Y
Nagoya journal of medical science 2023 Aug;85(3):528-541

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Lipid-accumulated reactive astrocytes promote disease progression in epilepsy.

Chen ZP, Wang S, Zhao X, Fang W, Wang Z, Ye H, Wang MJ, Ke L, Huang T, Lv P, Jiang X, Zhang Q, Li L, Xie ST, Zhu JN, Hang C, Chen D, Liu X, Yan C
Nature neuroscience 2023 Apr;26(4):542-554

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LncRNA Gm16638-201 Inhibits the 14-3-3Ɛ Pathway in the Murine Prefrontal Cortex to Induce Depressive Behaviors.

Zhang T, Wang WL, Liu TJ, Lu S, Bian YC, Xiao R, Zhang CL
Biological & pharmaceutical bulletin 2022;45(11):1616-1626

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A comprehensive mouse brain acetylome-the cellular-specific distribution of acetylated brain proteins.

Ji Y, Chen Z, Cen Z, Ye Y, Li S, Lu X, Shao Q, Wang D, Ji J, Ji Q
Frontiers in cellular neuroscience 2022;16:980815

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Hypoxia-pretreated mesenchymal stem cell-derived exosomes-loaded low-temperature extrusion 3D-printed implants for neural regeneration after traumatic brain injury in canines.

Liu X, Wang J, Wang P, Zhong L, Wang S, Feng Q, Wei X, Zhou L
Frontiers in bioengineering and biotechnology 2022;10:1025138

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Injectable and reactive oxygen species-scavenging gelatin hydrogel promotes neural repair in experimental traumatic brain injury.

Zhang D, Chang R, Ren Y, He Y, Guo S, Guan F, Yao M
International journal of biological macromolecules 2022 Oct 31;219:844-863

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In situ forming and biocompatible hyaluronic acid hydrogel with reactive oxygen species-scavenging activity to improve traumatic brain injury repair by suppressing oxidative stress and neuroinflammation.

Zhang D, Ren Y, He Y, Chang R, Guo S, Ma S, Guan F, Yao M
Materials today. Bio 2022 Jun;15:100278

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Injectable hyaluronic acid hydrogel loaded with BMSC and NGF for traumatic brain injury treatment.

Wang L, Zhang D, Ren Y, Guo S, Li J, Ma S, Yao M, Guan F
Materials today. Bio 2022 Jan;13:100201

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Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

Zhu PP, Hung HF, Batchenkova N, Nixon-Abell J, Henderson J, Zheng P, Renvoisé B, Pang S, Xu CS, Saalfeld S, Funke J, Xie Y, Svara F, Hess HF, Blackstone C
Human molecular genetics 2022 Aug 23;31(16):2779-2795

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Serum Protein Biomarker Findings Reflective of Oxidative Stress and Vascular Abnormalities in Male, but Not Female, Collision Sport Athletes.

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Frontiers in neurology 2020;11:549624

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Control the fate of human umbilical cord mesenchymal stem cells with dual-enzymatically cross-linked gelatin hydrogels for potential applications in nerve regeneration.

Li J, Gao F, Ma S, Zhang Y, Zhang J, Guan F, Yao M
Journal of tissue engineering and regenerative medicine 2020 Sep;14(9):1261-1271

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Perinatal exposure to nonylphenol delayed myelination in offspring cerebellum.

Jiang Y, You M, Li S, Xu Y, Wang Y
Biochemical pharmacology 2020 Aug;178:114120

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Inhibiting endogenous tissue plasminogen activator enhanced neuronal apoptosis and axonal injury after traumatic brain injury.

Zhao JJ, Liu ZW, Wang B, Huang TQ, Guo D, Zhao YL, Song JN
Neural regeneration research 2020 Apr;15(4):667-675

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Mixed Lineage Kinase Domain-like Protein MLKL Breaks Down Myelin following Nerve Injury.

Ying Z, Pan C, Shao T, Liu L, Li L, Guo D, Zhang S, Yuan T, Cao R, Jiang Z, Chen S, Wang F, Wang X
Molecular cell 2018 Nov 1;72(3):457-468.e5

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An Important Function of Petrosiol E in Inducing the Differentiation of Neuronal Progenitors and in Protecting Them against Oxidative Stress.

Liu J, Wang L, Du Y, Liu S
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2017 Oct;4(10):1700089

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Achaete-Scute Homolog 1 Expression Controls Cellular Differentiation of Neuroblastoma.

Kasim M, Heß V, Scholz H, Persson PB, Fähling M
Frontiers in molecular neuroscience 2016;9:156

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Bone marrow mesenchymal stem cells repair spinal cord ischemia/reperfusion injury by promoting axonal growth and anti-autophagy.

Yin F, Meng C, Lu R, Li L, Zhang Y, Chen H, Qin Y, Guo L
Neural regeneration research 2014 Sep 15;9(18):1665-71

Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse brain tissue were subjected to SDS PAGE followed by western blot with 12998-1-AP(NEFL antibody) at dilution of 1:4000

Sample type

tissue

Immunocytochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunofluorescent analysis of NIH3T3 cells, using NEFL antibody 12998-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 12998-1-AP(NEFL antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human brain using 12998-1-AP(NEFL antibody) at dilution of 1:50 (under 40x lens)

Sample type

tissue