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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10827 - Provider product page
- Provider
- Abnova Corporation
- Product name
- NEFL monoclonal antibody, clone 1H3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant NEFL.
- Isotype
- IgG
- Antibody clone number
- 1H3
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using NEFL monoclonal antibody, clone 1H3 (Cat # MAB10827) against HeLa (1) and Jurkat (2) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using NEFL monoclonal antibody, clone 1H3 (Cat # MAB10827) against human NEFL (aa: 422-543) recombinant protein. (Expected MW is 62 kDa)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA measurement of NEFL monoclonal antibody, clone 1H3 (Cat # MAB10827).
- Validation comment
- ELISA
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells using NEFL monoclonal antibody, clone 1H3 (Cat # MAB10827) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung cancer (A) and human bladder cancer (B) tissues using NEFL monoclonal antibody, clone 1H3 (Cat # MAB10827) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of Jurkat cells using NEFL monoclonal antibody, clone 1H3 (Cat # MAB10827) (green) and negative control (red).
- Validation comment
- Flow Cytometry
