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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- RQ6261 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Neurofilament Antibody (-Light) / NF-L / NEFL
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Storage
- After reconstitution, the Neurofilament Light antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human SH-SY5Y, 2) rat brain and 3) mouse brain lysate with Neurofilament Light antibody. Predicted molecular weight: 62-68 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE mouse spinal cord with Neurofilament Light antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE rat spinal cord with Neurofilament Light antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human 293T cells with Neurofilament Light antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Neurofilament Light antibody.
