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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- STJA0003699 - Provider product page
- Provider
- St John's Laboratory
- Product name
- Anti-NEFL antibody [DA2] (STJA0003699)
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody anti-NEFL/NF-L is suitable for use in Western Blot and Immunofluorescence research applications.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
NA- Epitope
- NA
- Antibody clone number
- NA
- Vial size
- NA
- Concentration
- NA
- Storage
- Store at-20°C, and avoid repeated freeze-thaw cycles.
- Handling
- NA
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Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunofluorescence of a section of rat frontal cortex showing labeling of Neurofilament-L (cat. STJA0003699, 1:500, red) and labeling of GFAP ( cat. STJA0003659, 1:5000, green). The anti-NFL antibody labels cell bodies and processes of pyramidal neurons, as well as dendrites and axons of other neuronal cells. While the anti-GFAP antibody labels the network of glial cells.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
Supportive validation
- Submitted by
- St John's Laboratory (provider)
- Main image
- Experimental details
- Immunostaining of mixed cultured rat neurons stained with anti-NFL antibody (cat. STJA0003699, green, 1:100) and anti-alpha internexin.
- Sample type
- NA
- Validation comment
- NA
- Primary Ab dilution
- NA
- Other comments
- NA
- Secondary Ab
- NA
- Secondary Ab dilution
- NA
- Protocol
- NA
