MA5-47382

antibody from Invitrogen Antibodies

Targeting: NEFL CMT1F, CMT2E, NF68, NFL, PPP1R110

Western blot

Antibody data

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Validation data

Product number: MA5-47382 - Provider product page
Provider: Invitrogen Antibodies
Product name: NEFL Monoclonal Antibody (1B11)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Reactivity: Human, Mouse, Rat, Bovine, Porcine
Host: Mouse
Isotype: IgG
Antibody clone number: 1B11
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

NEFL protein structure - MA5-47382 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Chromogenic immunohistochemistry of NEFL in formalin fixed paraffin embedded brain stem section from a transgenic mouse model of ALS. Samples were stained with NEFL monoclonal antibody (Product # MA5-47382) using a dilution of 1:1,000. Detected in DAB (brown) following the Vector LabsM.O.M.® ImmPRESS® HRP method, without the antigen retrieval step. Hematoxylin (blue) was used as the counterstain. The NEFL antibody labels what are clearly degenerated axons, showing typical swollen, sinusoidal and discontinuous profiles. Note that under these conditions healthy axons are not stained.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of NEFL in coronal section of the spinal cord of a rat given a midline C4 contusion injury three days previously. Samples were stained with NEFL monoclonal antibody (Product # MA5-47382) in green. Costained with rabbit polyclonal antibody to neurofilament NF-L C-terminus in red. The NEFL antibody stains prominent aggregates of material concentrated in the lateral funiculi and the dorsal columns but seen in lesser amounts throughout the section. These are degenerating and degenerated axons damaged by the C4 lesion. The neurofilament NF-L C-terminus antibody binds the C-terminal "tail" region of NF-L which is absent or destroyed during degeneration, so the NEFL positive profiles are largely negative for neurofilament NF-L C-terminus antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of NEFL in cow cerebellum section. Samples were stained with NEFL monoclonal antibody (Product # MA5-47382) using a dilution of 1:2,000 (green). Costained with chicken pAb to VLP1 (Product # PA5-143584) dilution 1:2,000 in red. Blue is Hoechst staining of nuclear DNA. Small section of cow cerebellum was fixed in 4% paraformaldehyde for 3 days, cut to 45 μM, and free-floating sections. At this concentration the NEFL antibody labels dendrites and axons of neuronal cells in the granular layer (lower left) and prominent basket cell axons surrounding the large Purkinje neurons. The VLP1 antibody reveals protein expressed in granule cells and in synapses of the molecular layer of the cerebellum.