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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA014850 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA014850, RRID:AB_1854397
- Product name
- Anti-NEFL
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human NEFL, Gene description: neurofilament, light polypeptide, Alternative Gene Names: CMT1F, CMT2E, NF68, NFL, PPP1R110, Validated applications: WB, IHC, Uniprot ID: P07196, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Integrative genomic analyses identify neuroblastoma risk genes involved in neuronal differentiation
Tirelli M, Bonfiglio F, Cantalupo S, Montella A, Avitabile M, Maiorino T, Diskin S, Iolascon A, Capasso M
Human Genetics 2024;143(11):1293-1309
Human Genetics 2024;143(11):1293-1309
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Recombinant expression validation
- Main image
- Experimental details
- Western blot analysis in control (vector only transfected HEK293T lysate) and NEFL over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416829).
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using HPA014850 antibody. Corresponding NEFL RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
