MA5-11833

antibody from Invitrogen Antibodies

Targeting: CD24 CD24A

Immunocytochemistry

Antibody data

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Validation data

Product number: MA5-11833 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-CD24 Monoclonal Antibody (SN3b)
Antibody type: Monoclonal
Antigen: Other
Description: MA5-11833 targets CD24 in WB, ICC/IF, FACS and IHC (P) applications and shows reactivity with Human and Mouse samples. This antibody is not suitable for mouse BAF-3 cell lysates in Western blot analysis. The MA5-11833 immunogen is cell membrane antigen preparation isolated from NALM-1 human pre-B leukemia cell line.
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgM
Antibody clone number: SN3b
Vial size: 500 µL
Concentration: 0.8 mg/mL
Storage: 4° C

CD24 protein structure - MA5-11833 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD24 (green) showing staining in the membrane of MCF-7 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD24 monoclonal antibody (Product # MA5-11833) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD24 (green) showing staining in the membrane and cytoplasm of BAF-3 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD24 monoclonal antibody (Product # MA5-11833) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of CD24 (green) showing staining in the membrane and cytoplasm of Ramos cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD24 monoclonal antibody (Product # MA5-11833) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.