Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AF5247 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD24 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human CD24 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
NP_037362
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references CD24 regulates cancer stem cell (CSC)-like traits and a panel of CSC-related molecules serves as a non-invasive urinary biomarker for the detection of bladder cancer.
Arsenic promotes the COX2/PGE2-SOX2 axis to increase the malignant stemness properties of urothelial cells.
PMP22 Regulates Self-Renewal and Chemoresistance of Gastric Cancer Cells.
Ooki A, VandenBussche CJ, Kates M, Hahn NM, Matoso A, McConkey DJ, Bivalacqua TJ, Hoque MO
British journal of cancer 2018 Oct;119(8):961-970
British journal of cancer 2018 Oct;119(8):961-970
Arsenic promotes the COX2/PGE2-SOX2 axis to increase the malignant stemness properties of urothelial cells.
Ooki A, Begum A, Marchionni L, VandenBussche CJ, Mao S, Kates M, Hoque MO
International journal of cancer 2018 Jul 1;143(1):113-126
International journal of cancer 2018 Jul 1;143(1):113-126
PMP22 Regulates Self-Renewal and Chemoresistance of Gastric Cancer Cells.
Cai W, Chen G, Luo Q, Liu J, Guo X, Zhang T, Ma F, Yuan L, Li B, Cai J
Molecular cancer therapeutics 2017 Jun;16(6):1187-1198
Molecular cancer therapeutics 2017 Jun;16(6):1187-1198
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human CD24 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human CD24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5247) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD24 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.