MAB12804

antibody from Abnova Corporation

Targeting: H3C1 H3/A, H3FA, HIST1H3A

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB12804

Provider

Abnova Corporation

Product name

Pan Histone H3 monoclonal antibody, clone RM188

Antibody type

Monoclonal

Description

Rabbit monoclonal antibody raised against synthetic peptide of human Pan Histone H3.

Isotype

IgG

Antibody clone number

RM188

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: recombinant Histone H3.3, Lane 2: recombinant Histone H3.1, Lane 3: A375, Lane 4: HEK293, Lane 5: HeLa and Lane 6: SK-MEL-2 whole cell lysate with Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804) at 0.025 ug/mL working concentration, showed a band of recombinant Histone H3.3, recombinant Histone H3.1 and total Histone H3 in A375, HEK293, HeLa and SK-MEL-2 cells.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Sandwich ELISA analysis of HeLa whole cell lysate treated or untreated with Sodium Butyrate (NaBu) using Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12804). A: Using Pan Histone H3 monoclonal antibody, clone RM188 as the capture antibody at 1 ug/mL working concentration and biotinylated format of Histone H3 (acetyl K9) monoclonal antibody, clone RM161 (Cat # MAB12839) as the detection antibody at 1 ug/mL working concentration. B: Using Histone H3 (acetyl K79) monoclonal antibody, clone RM156 as the capture antibody at 5 ug/mL working concentration and biotinylated format of Pan Histone H3 monoclonal antibody, clone RM188 (Cat # MAB12837) as the detection antibody at 1 ug/mL working concentration.

Validation comment

Sandwich ELISA