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- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- TA347157 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal H3K27ac Antibody
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal H3K27ac Antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- HIST1H3A
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 50 µg
- Concentration
- 1 ug/ul
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Cross reactivity of the antibody against H3K27ac To test the specificity an ELISA was performed using a serial dilution of the antibody against H3K27ac. The wells were coated with peptides containing the unmodified H3K27 region as well as the acetylated H3K27 and the acetylated H3K9. Image shows a high specificity of the antibody for the peptide containing the modification of interest.
- Validation comment
- ELISA
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HeLa cells were stained with the antibody against H3K27ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27ac antibody (left) diluted 1:500 in blocking solution followed by an anti-mouse antibody conjugated to Alexa594. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
- Validation comment
- IF
