815504

antibody from BioLegend

Targeting: H3C1 H3/A, H3FA, HIST1H3A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

815504

Provider

BioLegend

Product name

HRP anti-Histone H3 Dimethyl (Lys9)

Antibody type

Monoclonal

Reactivity

Human, Rat

Host

Mouse

Conjugate

Horseradish Peroxidase

Antibody clone number

5E5-G5

Vial size

100 µg

Concentration

0.50 mg/mL

Storage

-20°C

Handling

Blue Ice

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Western blot of HRP anti-Histone H3 Dimethyl (Lys9) antibody (5E5-G5). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate, Lane 3: 10 µg of mouse brain lysate, Lane 4: 10 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. Enhanced chemiluminescence was used as the detection system. * The predicted molecular weight of dimethyl Histone H3 is at 17 kD. ** The possible homodimers are at 35 kD (Antigen References #1 and #2)

Conjugate

Horseradish Peroxidase