BS2145

antibody from Bioworld Technology, Inc

Targeting: DCP1A HSA275986, SMAD4IP1, SMIF

Western blot (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

BS2145

Provider

Bioworld Technology, Inc

Proper citation

Bioworld Technology Cat#BS2145, RRID:AB_1662797

Product name

SMIF (S133) polyclonal antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide, corresponding to amino acids 102-150 of Human SMIF.

Description

Signal transduction of TGF-ÉÇ superfamily members is regulated by Smad proteins. In particular, Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-ÉÇ-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-intereacting protein) and Smad4 complex with TGF-ÉÇ and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through a EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-ÉÇ-induced nuclear translocation of SMIF (1). The human SMIF gene is ubiquitously expressed and encodes a protein with a relative molecular mass of 70 kDa.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100ul

Concentration

1 mg/ml

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.