Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-75261 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DCP1A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DCP1A in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: HepG2 whole cell lysate (40 µg), Lane 3: H1792 whole cell lysate (40 µg), Lane 4: AML-12 whole cell lysate (40 µg), Lane 5: PC12 whole cell lysate (40 µg). Samples were incubated with DCP1A polyclonal antibody (Product # PA5-75261) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of DCP1A in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: HepG2 whole cell lysate (40 µg), Lane 3: H1792 whole cell lysate (40 µg), Lane 4: AML-12 whole cell lysate (40 µg), Lane 5: PC12 whole cell lysate (40 µg). Samples were incubated with DCP1A polyclonal antibody (Product # PA5-75261) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of DCP1A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with DCP1A Polyclonal Antibody(Product # PA5-75261) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034), (1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DCP1A in paraffin-embedded human liver carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with DCP1A polyclonal antibody (Product # PA5-75261) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.