500-P268BT-1MG

antibody from Invitrogen Antibodies

Targeting: DEFB104A DEFB-4, DEFB104, DEFB4

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P268BT-1MG

Provider

Invitrogen Antibodies

Product name

DEFB104A Polyclonal Antibody, Biotin, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: EFELDRICGY GTARCRKKCR SQEYRIGRCP NTYACCLRKW DESLLNRTKP. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Humn BD-4. Anti-Human BD-4-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hBD-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BD-4 (500-P268) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-4. Western Blot: To detect hBD-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P268BT-1MG will be provided as 2 x 500 µg

Reactivity

Human

Host

Rabbit

Conjugate

Biotin

Vial size

2 x 500 µg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hBD-4 by Western Blot analysis DEFB104A Polyclonal Antibody, Biotin (Product # 500-P268BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Conjugate

Biotin

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot: To detect hBD-4 by Western Blot analysis DEFB104A Polyclonal Antibody, Biotin (Product # 500-P268BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hBD-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Conjugate

Biotin

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect hBD-4 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of DEFB104A Polyclonal Antibody, Biotin (Product # 500-P268BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech DEFB104A Polyclonal Antibody (Product # 500-P268-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hBD-4.

Conjugate

Biotin