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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [3]
- Immunoprecipitation [3]
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- Product number
- LS-C197606 - Provider product page
- Provider
- LSBio
- Product name
- p56lck / LCK Antibody (clone 3F7-F5) LS-C197606
- Antibody type
- Monoclonal
- Description
- Purified from ascites by Protein A
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3F7-F5
- Storage
- Store at -20°C or lower. Aliquot to avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- LCK monoclonal antibody (M01), clone 3F7-F5 Western Blot analysis of LCK expression in Jurkat.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot analysis of LCK expression in transfected 293T cell line by LCK monoclonal antibody (M01), clone 3F7-F5.Lane 1: LCK transfected lysate(59.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection limit for recombinant GST tagged LCK is approximately 0.1 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged LCK is approximately 0.1 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged LCK is approximately 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitation of LCK transfected lysate using anti-LCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LCK rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of LCK transfected lysate using anti-LCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LCK rabbit polyclonal antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitation of LCK transfected lysate using anti-LCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LCK rabbit polyclonal antibody.
