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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- AM20343PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM20343PU-N, RRID:AB_10755046
- Product name
- anti IKBKE / IKKE (175-188)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide corresponding to amino acids 175-188 (GTEEYLHPDMYERAC) of Human IKKe/IKKi protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 72B587
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of IKKe in Jurkat cell lysate using AM20343PU-N at 2 µg/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Skin, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Testis, Human: Formalin-Fixed, Paraffin-Embedded (FFPE)
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of IKKe in 10e6 NIH 3T3 cells using 0.1 ug of AM20343PU-N. Shaded histogram represents cells without antibody; green represents isotype control; red represents anti-IKKg antibody. Goat anti-mouse IgG FITC secondary antibody was used for this test.
