PA5-36233

antibody from Invitrogen Antibodies

Targeting: ACTN3

Western blot

Antibody data

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Validation data

Product number: PA5-36233 - Provider product page
Provider: Invitrogen Antibodies
Product name: alpha Actinin 3 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody detects endogenous protein at a molecular weight of 103 kDa. It does not cross-react with other alpha-actinin isoforms. Purity is >95% by SDS-PAGE.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ACTN3 protein structure - PA5-36233 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of alpha Actinin 3 in paraffin-embedded human tonsil carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of alpha Actinin 3 in paraffin-embedded human lung carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of alpha Actinin 3 in paraffin-embedded human colon carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of alpha Actinin 3 in paraffin-embedded human kidney carcinoma tissue (cytoplasmic and nucleus staining) and negative control (right, with PBS only). Samples were incubated with alpha Actinin 3 polyclonal antibody (Product # PA5-36233) at a dilution of 1:50, followed by goat Anti-Rabbit IgG-biotin and avidin peroxidase.