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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [4]
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- Product number
- R32494 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- ACTN3 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific ACTN3 antibody is suitable for use in Western blot/Immunohistochemistry/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the ACTN3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) rat skeletal muscle, 2) mouse skeletal muscle and 3) human HT080 lysate with ACTN3 antibody at 0.5ug/ml. Predicted molecular weight ~103 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HT1080, 2) rat skeletal muscle, 3) rat skeletal muscle, 4) mouse skeletal muscle and 5) mouse skeletal muscle tissue lysate with ACTN3 antibody at 0.5ug/ml. Predicted molecular weight ~103 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lung cancer tissue with ACTN3 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse skeletal muscle with ACTN3 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE rat skeletal muscle with ACTN3 antibody at 1ug/ml. HIER: steam sections in pH6 citrate buffer for 20 min.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE mouse skeletal muscle tissue with ACTN3 antibody (green) and DAPI nuclear stain (blue). HIER: steam section in pH8 EDTA buffer for 20 min.
