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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- MA5-27545 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ORAI1 (extracellular) Monoclonal Antibody (3F11/D10/B9)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Immunogen sequence: SSVSALKPG DWVIPANAGL GTWRTEAVFS EEALIGIPKD IPLQSAATLG VNPCTAYRML VDFEQLQPGD SVIQNASNSG VGQAVIQIAS ALRLKTINVV RDRPDIKKLT DRLKDLGADY VLTEEELRMP ETKTIFKDLP LPRLALNCVG GKSSTELLRH LAPGGTMVTY GGMAKQPVTA SVSLLIFKDL KLRGFWLSQW KKNHSPDEFK ELILTLCNLI RQGRLTAPSC SEVPLQGYQQ ALEASMKPFV SSKQILTM (117-373 aa encoded by BC003864)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- 3F11/D10/B9
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using 1) HEK-293 cells transfected with human Orai1, and 2) HEK-293 cells transfected with control vector with ORAI1 (extracellular) polyclonal antibody (Product # MA5-27545) using a dilution of 1:200.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ORAI1 (extracellular) in HEK-293 cells expressing Orai1. Samples were probed with ORAI1 (extracellular) monoclonal antibody (Product # MA5-27545). A) Extracellular stain of cells (red). B) Image of cells transmitted with empty vector.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of ORAI1 (extracellular) in HEK-293 cells expressing Orai1. Samples were probed with ORAI1 (extracellular) monoclonal antibody (Product # MA5-27545). A) Extracellular stain of cells (red). B) Image of cells transmitted with empty vector.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of Jurkat cells using monoclonal ORAI1 (extracellular) antibody (Product # MA5-27545) with a dilution of 5-10 µg/0.5-1x10^6cells.
