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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [2]
- Flow cytometry [1]
- Other assay [1]
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- Product number
- PA5-109270 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ORAI1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: PC-3M, LOVO, MCF-7, human skin tissue, human esophagus tissue.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
Submitted references Orai1 downregulation causes proliferation reduction and cell cycle arrest via inactivation of the Ras-NF-κB signaling pathway in osteoblasts.
Guo Y, Fan J, Liu S, Hao D
BMC musculoskeletal disorders 2022 Apr 11;23(1):347
BMC musculoskeletal disorders 2022 Apr 11;23(1):347
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Orai1 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Orai1 polyclonal antibody (Product # PA5-109270), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Orai1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Orai1 polyclonal antibody (Product # PA5-109270), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of Orai1 in MCF-7 cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with Orai1 polyclonal antibody (Product # PA5-109270) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- The expression of Orai1 is inhibited by Orai1 siRNA in MC3T3-E1 cells. a Orai1 protein levels were examined by immunofluorescence analysis in MC3T3-E1 cells transfected with either control siRNA or Orai1 siRNA (Scale =100 mum). b Orai1 protein levels were examined by western blot analysis in MC3T3-E1 cells transfected with either control siRNA or Orai1 siRNA. c The Orai1 mRNA levels were examined by real-time PCR in MC3T3-E1 cells transfected with either control siRNA or Orai1 siRNA. Data are presented as means +- SD from three independent experiments. * p < 0.05 as compared with the control
