Explore
Validate
Learn500-P125G-100UG
antibody from Invitrogen Antibodies
Targeting: CCL16
CKb12, HCC-4, LCC-1, LEC, LMC, Mtn-1, NCC-4, SCYA16, SCYL4
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 500-P125G-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCL16 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human LEC (CCL16). Anti-Human LEC (CCL16)-specific antibody was purified by affinity chromatography employing an immobilized Human LEC (CCL16) matrix. Sandwich ELISA: To detect Human LEC (CCL16) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human LEC (CCL16) (500-P125GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human (CCL16) LEC. Western Blot: To detect Human LEC (CCL16) by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human LEC (CCL16) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Goat
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Tumor rejection and immune memory elicited by locally released LEC chemokine are associated with an impressive recruitment of APCs, lymphocytes, and granulocytes.
LEC induces chemotaxis and adhesion by interacting with CCR1 and CCR8.
Giovarelli M, Cappello P, Forni G, Salcedo T, Moore PA, LeFleur DW, Nardelli B, Di Carlo E, Lollini PL, Ruben S, Ullrich S, Garotta G, Musiani P
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 15;164(6):3200-6
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 15;164(6):3200-6
LEC induces chemotaxis and adhesion by interacting with CCR1 and CCR8.
Howard OM, Dong HF, Shirakawa AK, Oppenheim JJ
Blood 2000 Aug 1;96(3):840-5
Blood 2000 Aug 1;96(3):840-5
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human LEC (CCL16) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL16 Polyclonal Antibody (Product # 500-P125G-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL16 Polyclonal Antibody, Biotin (Product # 500-P125GBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human (CCL16) LEC.
