500-P125G-1MG

antibody from Invitrogen Antibodies

Targeting: CCL16 CKb12, HCC-4, LCC-1, LEC, LMC, Mtn-1, NCC-4, SCYA16, SCYL4

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

500-P125G-1MG

Provider

Invitrogen Antibodies

Product name

CCL16 Polyclonal Antibody, PeproTech®

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

AA Sequence of recombinant protein: QPKVPEWVNT PSTCCLKYYE KVLPRRLVVG YRKALNCHLP AIIFVTKRNR EVCTNPNDDW VQEYIKDPNL PLLPTRNLST VKIITAKNGQ PQLLNSQ. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human LEC (CCL16). Anti-Human LEC (CCL16)-specific antibody was purified by affinity chromatography employing an immobilized Human LEC (CCL16) matrix. Sandwich ELISA: To detect Human LEC (CCL16) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human LEC (CCL16) (500-P125GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human (CCL16) LEC. Western Blot: To detect Human LEC (CCL16) by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human LEC (CCL16) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Reactivity

Human

Host

Goat

Vial size

1 mg

Concentration

0.1-1.0 mg/mL

Storage

-20°C

References

Submitted references

Tumor rejection and immune memory elicited by locally released LEC chemokine are associated with an impressive recruitment of APCs, lymphocytes, and granulocytes.

Giovarelli M, Cappello P, Forni G, Salcedo T, Moore PA, LeFleur DW, Nardelli B, Di Carlo E, Lollini PL, Ruben S, Ullrich S, Garotta G, Musiani P
Journal of immunology (Baltimore, Md. : 1950) 2000 Mar 15;164(6):3200-6

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LEC induces chemotaxis and adhesion by interacting with CCR1 and CCR8.

Howard OM, Dong HF, Shirakawa AK, Oppenheim JJ
Blood 2000 Aug 1;96(3):840-5

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Sandwich ELISA: To detect Human LEC (CCL16) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL16 Polyclonal Antibody (Product # 500-P125G-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL16 Polyclonal Antibody, Biotin (Product # 500-P125GBT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human (CCL16) LEC.