AGA-018-25UL

antibody from Invitrogen Antibodies

Targeting: GABRQ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AGA-018-25UL

Provider

Invitrogen Antibodies

Product name

GABA(A) theta Receptor (GABRQ) (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and Human CCF-STGI brain astrocytoma cell lysate (lanes 3 and 6): - 1-3. Anti-GABA (A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018), (1:500).4-6. Anti-GABA (A) θ Receptor (GABRQ) (extracellular) Antibody , preincubated with GABA (A) θ Receptor/GABRQ (extracellular) Blocking Peptide (#BLP-GA018).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain membrane (lanes 2 and 5) and Human CCF-STGI brain astrocytoma cell lysate (lanes 3 and 6): - 1-3. Anti-GABA (A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018), (1:500).4-6. Anti-GABA (A) θ Receptor (GABRQ) (extracellular) Antibody , preincubated with GABA (A) θ Receptor/GABRQ (extracellular) Blocking Peptide (#BLP-GA018).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of GABA (A) θ receptor in rat PC12 cells - Cell surface detection of GABRQ in intact living rat Pheochromocytoma (PC12) cells. A. Extracellular staining of cells using Anti-GABA (A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018), (1:50), (red). B. Merge of A with the live view of the cell.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of GABA (A) θ receptor in rat hypothalamus - Immunohistochemical staining of rat hypothalamus using Anti-GABA (A) θ Receptor (GABRQ) (extracellular) Antibody (#AGA-018). A.GABRQ staining (red) is detected in the mammillary nucleus which is part of the posterior hypothalamus (arrows demarcate nucleus border). B. Nuclear staining using DAPI as the counterstain (blue). C. Merge images of A and B.