STJA0003186

antibody from St John's Laboratory

Targeting: CCL14 CKb1, HCC-1, HCC-3, MCIF, NCC-2, SCYA14, SCYL2

Western blot

ELISA

Antibody data

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Validation data

Product number: STJA0003186 - Provider product page
Provider: St John's Laboratory
Product name: Anti-HCC-1 antibody (STJA0003186)
Antibody type: Polyclonal
Description: Rabbit polyclonal antibody anti-HCC-1 is suitable for use in ELISA and Western Blot research applications.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: NA
Epitope: NA
Antibody clone number: NA
Vial size: NA
Concentration: NA
Storage: Lyophilized antibody is stable at-20°C for up to 1 year from date of receipt. Upon reconstitution, store in aliquots at 2-8°C for one month or at-20°C for 6 months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Handling: NA

CCL14 protein structure - STJA0003186 shown in red.

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: 96-well maxisorp plates were coated with the capture antibody overnight, then blocked with 5% FCS in PBS for 1 hour, then washed. Then, the protein standard was added, and plates were incubated for 2 hrs at 37°C. Plates were washed and incubated with a biotinylated detection antibody for 1 hour at 37°C. After washing, plates were incubated with HRP-conjugated streptavidin for 30 min at room temperature and washed again. Plates were developed using the tetramethylbenzidine peroxidase substrate system and absorbance was measured at 450-615 nm using an automated plate reader.
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA

Supportive validation

Submitted by: St John's Laboratory (provider)
Main image
Experimental details: The Recombinant immunogen was migrated by 12% SDS-PAGE and transferred to a 0. 2 um PVDF membrane. After blocking the membrane in blocking buffer (5% milk powder in 20 mM Tris-HCl pH 7. 5, 500 mM NaCl, 0. 1% (v/v) Tween 20, the membrane was incubated with primary antibody ( at 4°C overnight. Peroxidase-linked secondary anti-rabbit were used to detect the bound primary antibodies. Enhanced chemiluminescence (ECL) reagents were used to visualize
Sample type: NA
Validation comment: NA
Primary Ab dilution: NA
Other comments: NA
Secondary Ab: NA
Secondary Ab dilution: NA
Protocol: NA
Appendix: NA