Explore
Validate
Learn500-P106-1MG
antibody from Invitrogen Antibodies
Targeting: CCL14
CKb1, HCC-1, HCC-3, MCIF, NCC-2, SCYA14, SCYL2
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 500-P106-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CCL14 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: TESSSRGPYH PSECCFTYTT YKIPRQRIMD YYETNSQCSK PGIVFITKRG HSVCTNPSDK WVQDYIKDMK EN. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human HCC-1 (CCL14). Anti-Human HCC-1 (CCL14)-specific antibody was purified by affinity chromatography employing an immobilized Human HCC-1 (CCL14) matrix. Sandwich ELISA: To detect Human HCC-1 (CCL14) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human HCC-1 (CCL14) (500-P106Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human HCC-1 (CCL14). Western Blot: To detect Human HCC-1 (CCL14) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human HCC-1 (CCL14) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 1 mg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references PARC/CCL18 is a plasma CC chemokine with increased levels in childhood acute lymphoblastic leukemia.
Struyf S, Schutyser E, Gouwy M, Gijsbers K, Proost P, Benoit Y, Opdenakker G, Van Damme J, Laureys G
The American journal of pathology 2003 Nov;163(5):2065-75
The American journal of pathology 2003 Nov;163(5):2065-75
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human HCC-1 (CCL14) by Western Blot analysis CCL14 Polyclonal Antibody (Product # 500-P106-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human HCC-1 (CCL14) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human HCC-1 (CCL14) by Western Blot analysis CCL14 Polyclonal Antibody (Product # 500-P106-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human HCC-1 (CCL14) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human HCC-1 (CCL14) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL14 Polyclonal Antibody (Product # 500-P106-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL14 Polyclonal Antibody, Biotin (Product # 500-P106BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human HCC-1 (CCL14).
