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LearnMAB363
antibody from R&D Systems
Targeting: CCL15
HCC-2, HMRP-2B, Lkn-1, MIP-1d, MIP-5, NCC-3, SCYA15, SCYL3
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB363 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CCL15/MIP-1 delta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CCL15/MIP-1 delta in ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q16663.2- Isotype
- IgG
- Antibody clone number
- 52130
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Expression and regulation of CCL15 by human airway smooth muscle cells.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Joubert P, Lajoie-Kadoch S, Wellemans V, Létuvé S, Tulic MK, Halayko AJ, Hamid Q
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2012 Jan;42(1):85-94
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2012 Jan;42(1):85-94
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Haringman JJ, Smeets TJ, Reinders-Blankert P, Tak PP
Annals of the rheumatic diseases 2006 Mar;65(3):294-300
Annals of the rheumatic diseases 2006 Mar;65(3):294-300
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL15/MIP-1 delta and Neutral-ization by Human CCL15/ MIP-1 delta Antibody. Recombinant Human CCL15/ MIP-1 delta (Catalog # 363-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/ MIP-1 delta (16 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL15/MIP-1 delta Mono-clonal Antibody (Catalog # MAB363). The ND50 is typically 3-15 µg/mL.
