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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Flow cytometry [3]
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- Product number
- GTX116018 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116018, RRID:AB_10621655
- Product name
- CD45 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Rat, Rabbit
- Host
- Rabbit
Submitted references Targeting P(2)X(7) receptor for the treatment of central post-stroke pain in a rodent model.
Differential protein expression between chondrogenic differentiated MSCs, undifferentiated MSCs and adult chondrocytes derived from Oryctolagus cuniculus in vitro.
Kuan YH, Shih HC, Tang SC, Jeng JS, Shyu BC
Neurobiology of disease 2015 Jun;78:134-45
Neurobiology of disease 2015 Jun;78:134-45
Differential protein expression between chondrogenic differentiated MSCs, undifferentiated MSCs and adult chondrocytes derived from Oryctolagus cuniculus in vitro.
Tay LX, Lim CK, Mansor A, Kamarul T
International journal of medical sciences 2014;11(1):24-33
International journal of medical sciences 2014;11(1):24-33
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T 5% SDS PAGE GTX116018 diluted at 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Raji whole cell and membrane extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with CD45 antibody (GTX116018) diluted at 1:1000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC D3, using CD45(GTX116018) antibody at 1:200 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CD45 antibody detects CD45 protein at cell membrane by immunofluorescent analysis.Sample: THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: CD45 protein stained by CD45 antibody (GTX116018) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CD45 antibody (GTX116018) detects CD45 protein by flow cytometry analysis. Sample: mouse splenocytes cell fixed in 4% paraformaldehyde at 4?C for 15 min. Black: Unlabelled sample was used as a control. Red: CD45 antibody (GTX116018) dilution: 1:50. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CD45 antibody (GTX116018) detects CD45 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD45 antibody (GTX116018) dilution: 1:50.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- CD45 antibody (GTX116018) detects CD45 protein by flow cytometry analysis. Sample: THP-1 cell. Black: Unlabelled sample was used as a control. Red: CD45 antibody (GTX116018) dilution: 1:50.
