Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- AF114 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD45 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CD45 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human CD45 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
NP_035340
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Type I interferons instigate fetal demise after Zika virus infection.
STAT5A/B Blockade Sensitizes Prostate Cancer to Radiation through Inhibition of RAD51 and DNA Repair.
Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion.
Prevention of C5aR1 signaling delays microglial inflammatory polarization, favors clearance pathways and suppresses cognitive loss.
Immunization with Bacillus Calmette-Guérin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain.
Metabolic origins of spatial organization in the tumor microenvironment.
CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis.
Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this.
Downstream Toll-like receptor signaling mediates adaptor-specific cytokine expression following focal cerebral ischemia.
Serine protease inhibition reduces post-ischemic granulocyte recruitment in mouse intestine.
Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling.
Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling.
Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells.
FGF2-dependent neovascularization of subcutaneous Matrigel plugs is initiated by bone marrow-derived pericytes and macrophages.
Yockey LJ, Jurado KA, Arora N, Millet A, Rakib T, Milano KM, Hastings AK, Fikrig E, Kong Y, Horvath TL, Weatherbee S, Kliman HJ, Coyne CB, Iwasaki A
Science immunology 2018 Jan 5;3(19)
Science immunology 2018 Jan 5;3(19)
STAT5A/B Blockade Sensitizes Prostate Cancer to Radiation through Inhibition of RAD51 and DNA Repair.
Maranto C, Udhane V, Hoang DT, Gu L, Alexeev V, Malas K, Cardenas K, Brody JR, Rodeck U, Bergom C, Iczkowski KA, Jacobsohn K, See W, Schmitt SM, Nevalainen MT
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Apr 15;24(8):1917-1931
Clinical cancer research : an official journal of the American Association for Cancer Research 2018 Apr 15;24(8):1917-1931
Molecular characterization of the transition from acute to chronic kidney injury following ischemia/reperfusion.
Liu J, Kumar S, Dolzhenko E, Alvarado GF, Guo J, Lu C, Chen Y, Li M, Dessing MC, Parvez RK, Cippà PE, Krautzberger AM, Saribekyan G, Smith AD, McMahon AP
JCI insight 2017 Sep 21;2(18)
JCI insight 2017 Sep 21;2(18)
Prevention of C5aR1 signaling delays microglial inflammatory polarization, favors clearance pathways and suppresses cognitive loss.
Hernandez MX, Jiang S, Cole TA, Chu SH, Fonseca MI, Fang MJ, Hohsfield LA, Torres MD, Green KN, Wetsel RA, Mortazavi A, Tenner AJ
Molecular neurodegeneration 2017 Sep 18;12(1):66
Molecular neurodegeneration 2017 Sep 18;12(1):66
Immunization with Bacillus Calmette-Guérin (BCG) alleviates neuroinflammation and cognitive deficits in APP/PS1 mice via the recruitment of inflammation-resolving monocytes to the brain.
Zuo Z, Qi F, Yang J, Wang X, Wu Y, Wen Y, Yuan Q, Zou J, Guo K, Yao ZB
Neurobiology of disease 2017 May;101:27-39
Neurobiology of disease 2017 May;101:27-39
Metabolic origins of spatial organization in the tumor microenvironment.
Carmona-Fontaine C, Deforet M, Akkari L, Thompson CB, Joyce JA, Xavier JB
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 14;114(11):2934-2939
Proceedings of the National Academy of Sciences of the United States of America 2017 Mar 14;114(11):2934-2939
CREBH-FGF21 axis improves hepatic steatosis by suppressing adipose tissue lipolysis.
Park JG, Xu X, Cho S, Hur KY, Lee MS, Kersten S, Lee AH
Scientific reports 2016 Jun 15;6:27938
Scientific reports 2016 Jun 15;6:27938
Low-dose irradiation affects expression of inflammatory markers in the heart of ApoE -/- mice.
Mathias D, Mitchel RE, Barclay M, Wyatt H, Bugden M, Priest ND, Whitman SC, Scholz M, Hildebrandt G, Kamprad M, Glasow A
PloS one 2015;10(3):e0119661
PloS one 2015;10(3):e0119661
Runx1 is required for progression of CD41+ embryonic precursors into HSCs but not prior to this.
Liakhovitskaia A, Rybtsov S, Smith T, Batsivari A, Rybtsova N, Rode C, de Bruijn M, Buchholz F, Gordon-Keylock S, Zhao S, Medvinsky A
Development (Cambridge, England) 2014 Sep;141(17):3319-23
Development (Cambridge, England) 2014 Sep;141(17):3319-23
Downstream Toll-like receptor signaling mediates adaptor-specific cytokine expression following focal cerebral ischemia.
Famakin B, Mou Y, Spatz M, Lawal M, Hallenbeck J
Journal of neuroinflammation 2012 Jul 16;9:174
Journal of neuroinflammation 2012 Jul 16;9:174
Serine protease inhibition reduces post-ischemic granulocyte recruitment in mouse intestine.
Gobbetti T, Cenac N, Motta JP, Rolland C, Martin L, Andrade-Gordon P, Steinhoff M, Barocelli E, Vergnolle N
The American journal of pathology 2012 Jan;180(1):141-52
The American journal of pathology 2012 Jan;180(1):141-52
Protein-tyrosine phosphatase DEP-1 controls receptor tyrosine kinase FLT3 signaling.
Arora D, Stopp S, Böhmer SA, Schons J, Godfrey R, Masson K, Razumovskaya E, Rönnstrand L, Tänzer S, Bauer R, Böhmer FD, Müller JP
The Journal of biological chemistry 2011 Apr 1;286(13):10918-29
The Journal of biological chemistry 2011 Apr 1;286(13):10918-29
Schistosomiasis-induced experimental pulmonary hypertension: role of interleukin-13 signaling.
Graham BB, Mentink-Kane MM, El-Haddad H, Purnell S, Zhang L, Zaiman A, Redente EF, Riches DW, Hassoun PM, Bandeira A, Champion HC, Butrous G, Wynn TA, Tuder RM
The American journal of pathology 2010 Sep;177(3):1549-61
The American journal of pathology 2010 Sep;177(3):1549-61
Fetal liver hepatic progenitors are supportive stromal cells for hematopoietic stem cells.
Chou S, Lodish HF
Proceedings of the National Academy of Sciences of the United States of America 2010 Apr 27;107(17):7799-804
Proceedings of the National Academy of Sciences of the United States of America 2010 Apr 27;107(17):7799-804
FGF2-dependent neovascularization of subcutaneous Matrigel plugs is initiated by bone marrow-derived pericytes and macrophages.
Tigges U, Hyer EG, Scharf J, Stallcup WB
Development (Cambridge, England) 2008 Feb;135(3):523-32
Development (Cambridge, England) 2008 Feb;135(3):523-32
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD45 in Mouse Liver. CD45 was detected in immersion fixed paraffin-embedded sections of mouse liver using Goat Anti-Mouse CD45 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF114) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Kupffer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.