Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 83-0458-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD45RA Monoclonal Antibody (HI100), eVolve 605, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Notice: The eVolve line of antibody conjugates will be discontinued at the end of February, 2018 and replaced with our improved Invitrogen eBioscience Super Bright conjugated antibodies for flow cytometry. Individual eVovle catalog numbers will become unavailable as inventory depletes. This item has a corresponding Super Bright 600 format available please see Product # 63-9956-42. We will continue to offer a selection of Qdot conjugated antibodies. Description: The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of alpha and beta subunits and plays an important role in the presentation of peptides to CD4+ T lymphocytes. Applications Reported: This LN3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL per test. A test is defined as the amount of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. For best results when using antibodies conjugated to eVolve, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip. Laser/Filter Recommendation: eVolve 605 can be optimally excited with laser lines from 325-405 nm, and emits near 610 nm. We recommend using the violet (405 nm) laser line for excitation and a 595LP with a 605/40 or 610/20 bandpass filter, or equivalent, for detection. Fixation Recommendation: Antibodies conjugated to eVolve are compatible with eBioscience's fixation and permeabilization buffers. Excitation: 350-590 nm; Emission: 605 nm; Laser: Ultraviolet Laser, Violet Laser Storage and handling: For best results when using antibodies conjugated to eVolve, we recommend a quick spin of the vial before use and avoid touching the bottom of the vial with the pipet tip.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HI100
- Vial size
- 100 Tests
- Concentration
- 5 µl/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Atezolizumab in combination with bevacizumab enhances antigen-specific T-cell migration in metastatic renal cell carcinoma.
Wallin JJ, Bendell JC, Funke R, Sznol M, Korski K, Jones S, Hernandez G, Mier J, He X, Hodi FS, Denker M, Leveque V, Cañamero M, Babitski G, Koeppen H, Ziai J, Sharma N, Gaire F, Chen DS, Waterkamp D, Hegde PS, McDermott DF
Nature communications 2016 Aug 30;7:12624
Nature communications 2016 Aug 30;7:12624
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD45RO FITC (Product # 11-0457-42) and Anti-Human CD45RA eVolve™ 605. Cells in the lymphocyte gate were used for analysis.