NB500-319

antibody from Novus Biologicals

Targeting: PTPRC CD45, GP180, LCA, T200

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB500-319

Provider

Novus Biologicals

Proper citation

Novus Cat#NB500-319, RRID:AB_10001403

Product name

Mouse Monoclonal CD45 Antibody

Antibody type

Monoclonal

Description

Protein A purified. The antibody MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. HLDA III

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Human macrophages survive and adopt activated genotypes in living zebrafish.

Paul CD, Devine A, Bishop K, Xu Q, Wulftange WJ, Burr H, Daly KM, Lewis C, Green DS, Staunton JR, Choksi S, Liu ZG, Sood R, Tanner K
Scientific reports 2019 Feb 11;9(1):1759

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Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin.

Palchaudhuri R, Saez B, Hoggatt J, Schajnovitz A, Sykes DB, Tate TA, Czechowicz A, Kfoury Y, Ruchika F, Rossi DJ, Verdine GL, Mansour MK, Scadden DT
Nature biotechnology 2016 Jul;34(7):738-45

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Cabozantinib inhibits prostate cancer growth and prevents tumor-induced bone lesions.

Dai J, Zhang H, Karatsinides A, Keller JM, Kozloff KM, Aftab DT, Schimmoller F, Keller ET
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Feb 1;20(3):617-30

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Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation.

Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, Bemark M
Journal of leukocyte biology 2011 Jul;90(1):5-19

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Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement.

Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ
International immunology 2007 May;19(5):675-84

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Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules.

Cermák L, Símová S, Pintzas A, Horejsí V, Andera L
The Journal of biological chemistry 2002 Mar 8;277(10):7955-61

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CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes.

Ilangumaran S, Briol A, Hoessli DC
Blood 1998 May 15;91(10):3901-8

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Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).

Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I
Folia biologica 1988;34(1):23-34

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: CD45 Antibody (MEM-28) [NB500-319] - Staining of human small intestine (paraffin-embedded sections) with anti-CD45 (MEM-28), 10 ug/ml

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) PerCP.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) APC-CyTM7.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) Alexa Fluor (R) 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) PerCP-CyTM5.5.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) purified, GAM-APC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) FITC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Analysis using the Allophycocyanin conjugate of NB500-319. Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) APC

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) PE-CyTM5. Image from the Biotin version of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) Pacific BlueTM. Image from the Biotin version of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) PE-DyLight (R) 594. Image from the Biotin version of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) PE. Image from the Biotin version of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) PE-CyTM7. Image from the Biotin version of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) biotin. Image from the Biotin version of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) Alexa Fluor(R) 647 Image from the Biotin version of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) FITC. Image from the Biotin version of this antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood lymphocytes with anti-human CD45 (MEM-28) Alexa Fluor 700.