Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [16]
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Validation data
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- Product number
- NB500-319 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-319, RRID:AB_10001403
- Product name
- Mouse Monoclonal CD45 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. The antibody MEM-28 reacts with all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. HLDA III
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Human macrophages survive and adopt activated genotypes in living zebrafish.
Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin.
Cabozantinib inhibits prostate cancer growth and prevents tumor-induced bone lesions.
Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation.
Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement.
Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules.
CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes.
Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
Paul CD, Devine A, Bishop K, Xu Q, Wulftange WJ, Burr H, Daly KM, Lewis C, Green DS, Staunton JR, Choksi S, Liu ZG, Sood R, Tanner K
Scientific reports 2019 Feb 11;9(1):1759
Scientific reports 2019 Feb 11;9(1):1759
Non-genotoxic conditioning for hematopoietic stem cell transplantation using a hematopoietic-cell-specific internalizing immunotoxin.
Palchaudhuri R, Saez B, Hoggatt J, Schajnovitz A, Sykes DB, Tate TA, Czechowicz A, Kfoury Y, Ruchika F, Rossi DJ, Verdine GL, Mansour MK, Scadden DT
Nature biotechnology 2016 Jul;34(7):738-45
Nature biotechnology 2016 Jul;34(7):738-45
Cabozantinib inhibits prostate cancer growth and prevents tumor-induced bone lesions.
Dai J, Zhang H, Karatsinides A, Keller JM, Kozloff KM, Aftab DT, Schimmoller F, Keller ET
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Feb 1;20(3):617-30
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Feb 1;20(3):617-30
Pivotal advance: CD45RB glycosylation is specifically regulated during human peripheral B cell differentiation.
Koethe S, Zander L, Köster S, Annan A, Ebenfelt A, Spencer J, Bemark M
Journal of leukocyte biology 2011 Jul;90(1):5-19
Journal of leukocyte biology 2011 Jul;90(1):5-19
Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement.
Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ
International immunology 2007 May;19(5):675-84
International immunology 2007 May;19(5):675-84
Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules.
Cermák L, Símová S, Pintzas A, Horejsí V, Andera L
The Journal of biological chemistry 2002 Mar 8;277(10):7955-61
The Journal of biological chemistry 2002 Mar 8;277(10):7955-61
CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes.
Ilangumaran S, Briol A, Hoessli DC
Blood 1998 May 15;91(10):3901-8
Blood 1998 May 15;91(10):3901-8
Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I
Folia biologica 1988;34(1):23-34
Folia biologica 1988;34(1):23-34
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: CD45 Antibody (MEM-28) [NB500-319] - Staining of human small intestine (paraffin-embedded sections) with anti-CD45 (MEM-28), 10 ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) PerCP.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) APC-CyTM7.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) Alexa Fluor (R) 488.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) PerCP-CyTM5.5.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) purified, GAM-APC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) FITC.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Analysis using the Allophycocyanin conjugate of NB500-319. Surface staining of human peripheral blood cells with anti-human CD45 (MEM-28) APC
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) PE-CyTM5. Image from the Biotin version of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) Pacific BlueTM. Image from the Biotin version of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) PE-DyLight (R) 594. Image from the Biotin version of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) PE. Image from the Biotin version of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) PE-CyTM7. Image from the Biotin version of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) biotin. Image from the Biotin version of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) Alexa Fluor(R) 647 Image from the Biotin version of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood cells with anti-human CD45 (MEM-28) FITC. Image from the Biotin version of this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD45 Antibody (MEM-28) [NB500-319] - Staining of human peripheral blood lymphocytes with anti-human CD45 (MEM-28) Alexa Fluor 700.