Ab094902

antibody from Aladdin Scientific

Targeting: PTPRC CD45, GP180, LCA, T200

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab094902

Provider

Aladdin Scientific

Product name

CD45 Mouse mAb

Antibody type

Monoclonal

Description

Mouse anti Human CD45 Antibody, Monoclonal (HI30), could be used for Flow and so on.Application:Flow: < 0.5 _g/100w cellsProtein FunctionProtein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

HI30

Vial size

100_g,10_g,1mg,50_g

Concentration

Lot by Lot

Storage

Shipped at 4¡C. Store at 4¡C long term (36 months). Upon delivery aliquot.

Flow cytometry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

CD45 Mouse mAb (Ab094902) - Flow Cytometry Flow analysis of CD45 (red line) in Raji cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

CD45 Mouse mAb (Ab094902) - Flow Cytometry Flow analysis of CD45 (red line) in Jurkat cells. Cells were stained with CD45 Mouse mAb (Ab007182) at 0.1 μg/mL for 1 hour at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab156254) at a dilution of 1/1000 for 30 minutes at 4°C in the dark. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

CD45 Mouse mAb (Ab094902) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 with CD45 Mouse mAb (Ab094902) conjugated with biotin (right panel) at 0.1 μg/mL for 1 hour at 4°C, compared with mouse IgG (Ab170221) conjugated with biotin - Isotype Control (left panel) and detected with SA-PE (rp156250).

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

CD45 Mouse mAb (Ab094902) - Flow Cytometry Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD45 (red) with CD45 Mouse mAb (Ab094902) conjugated with biotin at 0.1 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156250). Blue - Isotype control, mouse IgG (Ab170221) conjugated with biotin. Black - Unlabelled control, cells without incubation with primary antibody.