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Western blot
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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-B2965 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ CHGA / Chromogranin A Antibody (aa19-131, clone 3A5) LS-B2965
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 3A5
- Storage
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Cell lysates of A549, 293T and mouse kidney (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Chromogranin A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Cell lysates of A549, 293T and mouse kidney (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Chromogranin A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-CHGA / Chromogranin A antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Anti-CHGA / Chromogranin A antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
