Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- AM31705PU-N - Provider product page
- Provider
- OriGene
- Product name
- Chromogranin A (CHGA) (19-131) mouse monoclonal antibody, clone 3A5, Purified
- Antibody type
- Monoclonal
- Description
- Chromogranin A (CHGA) (19-131) mouse monoclonal antibody, clone 3A5, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- CHGA
- Isotype
- IgG
- Antibody clone number
- 3A5
- Vial size
- 50 µg
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Cell lysates of A549, 293T and Mouse kidney (35 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Chromogranin A (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- CHGA antibody staining of Formalin-Fixed, Paraffin-Embedded Human Colon at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- CHGA antibody staining of Formalin-Fixed, Paraffin-Embedded Human Adrenal at 5 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
- Validation comment
- IHC