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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NBP1-86056 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86056, RRID:AB_11027016
- Product name
- Rabbit Polyclonal Chromogranin A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Chromogranin A antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Identification of a gene regulatory network associated with prion replication.
Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers.
Marbiah MM, Harvey A, West BT, Louzolo A, Banerjee P, Alden J, Grigoriadis A, Hummerich H, Kan HM, Cai Y, Bloom GS, Jat P, Collinge J, Klöhn PC
The EMBO journal 2014 Jul 17;33(14):1527-47
The EMBO journal 2014 Jul 17;33(14):1527-47
Scalable in situ hybridization on tissue arrays for validation of novel cancer and tissue-specific biomarkers.
Kiflemariam S, Andersson S, Asplund A, Pontén F, Sjöblom T
PloS one 2012;7(3):e32927
PloS one 2012;7(3):e32927
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Chromogranin A Antibody [NBP1-86056] - Analysis in control (vector only transfected HEK293T lysate) and CHGA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Chromogranin A Antibody [NBP1-86056] - Analysis in human plasma.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP1-86056] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP1-86056] - Staining of human parathyroid gland shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Chromogranin A Antibody [NBP1-86056] - Staining in human parathyroid gland and liver tissues using anti-CHGA antibody. Corresponding CHGA RNA-seq data are presented for the same tissues.
