Antibody data
- Antibody Data
- Antigen structure
- References [30]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
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Validation data
Reference
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- Product number
- MA5-13093 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Chromogranin A Monoclonal Antibody (LK2H10), Biotin
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA5-13093 targets Chromogranin A in IHC (P), ICC/IF and WB applications and shows reactivity with Human, Non-human primate, Porcine, Rabbit, and Rat samples. The MA5-13093 immunogen is human pheochromocytoma.
- Reactivity
- Human, Rat, Porcine, Rabbit
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- LK2H10
- Vial size
- 500 µL
- Concentration
- 0.2 mg/mL
- Storage
- 4° C
Submitted references Bevacizumab plus octreotide and metronomic capecitabine in patients with metastatic well-to-moderately differentiated neuroendocrine tumors: the XELBEVOCT study.
Gastric histopathological findings and ghrelin expression in morbid obesity.
Human ASH-1 promotes neuroendocrine differentiation in androgen deprivation conditions and interferes with androgen responsiveness in prostate cancer cells.
Human papillomavirus-associated adenocarcinoma of the base of the tongue.
Tumor staging but not grading is associated with adverse clinical outcome in neuroendocrine tumors of the appendix: a retrospective clinical pathologic analysis of 138 cases.
HPV-associated neuroendocrine carcinoma of the oropharynx: a rare new entity with potentially aggressive clinical behavior.
EGF promotes neuroendocrine-like differentiation of prostate cancer cells in the presence of LY294002 through increased ErbB2 expression independent of the phosphatidylinositol 3-kinase-AKT pathway.
Airway basal cell vascular endothelial growth factor-mediated cross-talk regulates endothelial cell-dependent growth support of human airway basal cells.
Human achaete-scute homolog-1 expression in neuroendocrine breast carcinoma.
Extraventricular neurocytomas: a morphological and histogenetic consideration. A study of six cases.
Detection of bone marrow metastases of neuroblastoma with immunohistochemical staining of CD56, chromogranin A, and synaptophysin.
Chromogranin peptides in amyotrophic lateral sclerosis.
Extra-adrenal composite paraganglioma with ganglioneuroma component presenting as a pancreatic mass.
Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.
A large form of secretogranin III functions as a sorting receptor for chromogranin A aggregates in PC12 cells.
Basaloid adenocarcinoma. A new variant of pulmonary adenocarcinoma.
Primary neuroendocrine carcinoma of the breast: a case report.
Primary neuroendocrine carcinoma of the breast: a case report.
Laparoscopic resection of urinary bladder paraganglioma: a case report.
Two functional epitopes of pigment epithelial-derived factor block angiogenesis and induce differentiation in prostate cancer.
Pulmonary large cell carcinoma with rhabdoid phenotype.
Ovarian ependymoma.
CDX-2 homeobox gene product expression in neuroendocrine tumors: its role as a marker of intestinal neuroendocrine tumors.
CDX-2 homeobox gene product expression in neuroendocrine tumors: its role as a marker of intestinal neuroendocrine tumors.
Altered distribution of metaplastic Paneth, gastrin and pancreatic acinar cells in atrophic gastritic mucosa with endocrine cell lesions.
Altered distribution of metaplastic Paneth, gastrin and pancreatic acinar cells in atrophic gastritic mucosa with endocrine cell lesions.
Concurrent lymph node metastases of medullary and papillary thyroid carcinoma in a case with RET oncogene germline mutation.
Concurrent lymph node metastases of medullary and papillary thyroid carcinoma in a case with RET oncogene germline mutation.
Classification of low-grade neuroendocrine tumors of midgut and unknown origin.
Heparin-binding epidermal growth factor-like growth factor stimulates androgen-independent prostate tumor growth and antagonizes androgen receptor function.
Berruti A, Fazio N, Ferrero A, Brizzi MP, Volante M, Nobili E, Tozzi L, Bodei L, Torta M, D'Avolio A, Priola AM, Birocco N, Amoroso V, Biasco G, Papotti M, Dogliotti L
BMC cancer 2014 Mar 14;14:184
BMC cancer 2014 Mar 14;14:184
Gastric histopathological findings and ghrelin expression in morbid obesity.
Gündoğan M, Çalli Demırkan N, Tekın K, Aybek H
Turk patoloji dergisi 2013;29(1):19-26
Turk patoloji dergisi 2013;29(1):19-26
Human ASH-1 promotes neuroendocrine differentiation in androgen deprivation conditions and interferes with androgen responsiveness in prostate cancer cells.
Rapa I, Volante M, Migliore C, Farsetti A, Berruti A, Vittorio Scagliotti G, Giordano S, Papotti M
The Prostate 2013 Aug;73(11):1241-9
The Prostate 2013 Aug;73(11):1241-9
Human papillomavirus-associated adenocarcinoma of the base of the tongue.
Hanna J, Reimann JD, Haddad RI, Krane JF
Human pathology 2013 Aug;44(8):1516-23
Human pathology 2013 Aug;44(8):1516-23
Tumor staging but not grading is associated with adverse clinical outcome in neuroendocrine tumors of the appendix: a retrospective clinical pathologic analysis of 138 cases.
Volante M, Daniele L, Asioli S, Cassoni P, Comino A, Coverlizza S, De Giuli P, Fava C, Manini C, Berruti A, Papotti M
The American journal of surgical pathology 2013 Apr;37(4):606-12
The American journal of surgical pathology 2013 Apr;37(4):606-12
HPV-associated neuroendocrine carcinoma of the oropharynx: a rare new entity with potentially aggressive clinical behavior.
Kraft S, Faquin WC, Krane JF
The American journal of surgical pathology 2012 Mar;36(3):321-30
The American journal of surgical pathology 2012 Mar;36(3):321-30
EGF promotes neuroendocrine-like differentiation of prostate cancer cells in the presence of LY294002 through increased ErbB2 expression independent of the phosphatidylinositol 3-kinase-AKT pathway.
Cortés MA, Cariaga-Martinez AE, Lobo MV, Martín Orozco RM, Motiño O, Rodríguez-Ubreva FJ, Angulo J, López-Ruiz P, Colás B
Carcinogenesis 2012 Jun;33(6):1169-77
Carcinogenesis 2012 Jun;33(6):1169-77
Airway basal cell vascular endothelial growth factor-mediated cross-talk regulates endothelial cell-dependent growth support of human airway basal cells.
Curradi G, Walters MS, Ding BS, Rafii S, Hackett NR, Crystal RG
Cellular and molecular life sciences : CMLS 2012 Jul;69(13):2217-31
Cellular and molecular life sciences : CMLS 2012 Jul;69(13):2217-31
Human achaete-scute homolog-1 expression in neuroendocrine breast carcinoma.
Righi L, Rapa I, Votta A, Papotti M, Sapino A
Virchows Archiv : an international journal of pathology 2012 Apr;460(4):415-21
Virchows Archiv : an international journal of pathology 2012 Apr;460(4):415-21
Extraventricular neurocytomas: a morphological and histogenetic consideration. A study of six cases.
Agarwal S, Sharma MC, Sarkar C, Suri V, Jain A, Sharma MS, Ailawadhi P, Garg A, Mallick S
Pathology 2011 Jun;43(4):327-34
Pathology 2011 Jun;43(4):327-34
Detection of bone marrow metastases of neuroblastoma with immunohistochemical staining of CD56, chromogranin A, and synaptophysin.
Park SJ, Park CJ, Kim S, Jang S, Chi HS, Kim MJ, Im HJ, Seo JJ
Applied immunohistochemistry & molecular morphology : AIMM 2010 Jul;18(4):348-52
Applied immunohistochemistry & molecular morphology : AIMM 2010 Jul;18(4):348-52
Chromogranin peptides in amyotrophic lateral sclerosis.
Schrott-Fischer A, Bitsche M, Humpel C, Walcher C, Maier H, Jellinger K, Rabl W, Glueckert R, Marksteiner J
Regulatory peptides 2009 Jan 8;152(1-3):13-21
Regulatory peptides 2009 Jan 8;152(1-3):13-21
Extra-adrenal composite paraganglioma with ganglioneuroma component presenting as a pancreatic mass.
Inzani F, Rindi G, Tamborrino E, Cobelli R, Bordi C
Endocrine pathology 2009 Fall;20(3):191-5
Endocrine pathology 2009 Fall;20(3):191-5
Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.
Mukhopadhyay S, El-Zammar OA, Katzenstein AL
The American journal of surgical pathology 2009 Apr;33(4):487-95
The American journal of surgical pathology 2009 Apr;33(4):487-95
A large form of secretogranin III functions as a sorting receptor for chromogranin A aggregates in PC12 cells.
Han L, Suda M, Tsuzuki K, Wang R, Ohe Y, Hirai H, Watanabe T, Takeuchi T, Hosaka M
Molecular endocrinology (Baltimore, Md.) 2008 Aug;22(8):1935-49
Molecular endocrinology (Baltimore, Md.) 2008 Aug;22(8):1935-49
Basaloid adenocarcinoma. A new variant of pulmonary adenocarcinoma.
Marci V, Volante M, Cappia S, Righi L, Novello C, Scagliotti GV, Brambilla E, Papotti M
Virchows Archiv : an international journal of pathology 2007 Sep;451(3):729-36
Virchows Archiv : an international journal of pathology 2007 Sep;451(3):729-36
Primary neuroendocrine carcinoma of the breast: a case report.
Yaren A, Kelten C, Akbulut M, Teke Z, Duzcan E, Erdem E
Tumori 2007 Sep-Oct;93(5):496-8
Tumori 2007 Sep-Oct;93(5):496-8
Primary neuroendocrine carcinoma of the breast: a case report.
Yaren A, Kelten C, Akbulut M, Teke Z, Duzcan E, Erdem E
Tumori 2007 Sep-Oct;93(5):496-8
Tumori 2007 Sep-Oct;93(5):496-8
Laparoscopic resection of urinary bladder paraganglioma: a case report.
Dilbaz B, Bayoglu Y, Oral S, Cavusoglu D, Uluoglu O, Dilbaz S
Surgical laparoscopy, endoscopy & percutaneous techniques 2006 Feb;16(1):58-61
Surgical laparoscopy, endoscopy & percutaneous techniques 2006 Feb;16(1):58-61
Two functional epitopes of pigment epithelial-derived factor block angiogenesis and induce differentiation in prostate cancer.
Filleur S, Volz K, Nelius T, Mirochnik Y, Huang H, Zaichuk TA, Aymerich MS, Becerra SP, Yap R, Veliceasa D, Shroff EH, Volpert OV
Cancer research 2005 Jun 15;65(12):5144-52
Cancer research 2005 Jun 15;65(12):5144-52
Pulmonary large cell carcinoma with rhabdoid phenotype.
Yilmazbayhan D, Ates LE, Dilege S, Gulluoglu M, Tanju S, Kalayci G
Annals of diagnostic pathology 2005 Aug;9(4):223-6
Annals of diagnostic pathology 2005 Aug;9(4):223-6
Ovarian ependymoma.
Erdoğan G, Ozel E, Peştereli HE, Salar Z, Tirak B, Karaveli S
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2005 Apr;113(4):301-3
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2005 Apr;113(4):301-3
CDX-2 homeobox gene product expression in neuroendocrine tumors: its role as a marker of intestinal neuroendocrine tumors.
Barbareschi M, Roldo C, Zamboni G, Capelli P, Cavazza A, Macri E, Cangi MG, Chilosi M, Doglioni C
The American journal of surgical pathology 2004 Sep;28(9):1169-76
The American journal of surgical pathology 2004 Sep;28(9):1169-76
CDX-2 homeobox gene product expression in neuroendocrine tumors: its role as a marker of intestinal neuroendocrine tumors.
Barbareschi M, Roldo C, Zamboni G, Capelli P, Cavazza A, Macri E, Cangi MG, Chilosi M, Doglioni C
The American journal of surgical pathology 2004 Sep;28(9):1169-76
The American journal of surgical pathology 2004 Sep;28(9):1169-76
Altered distribution of metaplastic Paneth, gastrin and pancreatic acinar cells in atrophic gastritic mucosa with endocrine cell lesions.
Deveci MS, Deveci G
The Tohoku journal of experimental medicine 2004 Jan;202(1):13-22
The Tohoku journal of experimental medicine 2004 Jan;202(1):13-22
Altered distribution of metaplastic Paneth, gastrin and pancreatic acinar cells in atrophic gastritic mucosa with endocrine cell lesions.
Deveci MS, Deveci G
The Tohoku journal of experimental medicine 2004 Jan;202(1):13-22
The Tohoku journal of experimental medicine 2004 Jan;202(1):13-22
Concurrent lymph node metastases of medullary and papillary thyroid carcinoma in a case with RET oncogene germline mutation.
Papi G, Corrado S, Pomponi MG, Carapezzi C, Cesinaro A, LiVolsi VA
Endocrine pathology 2003 Fall;14(3):269-76
Endocrine pathology 2003 Fall;14(3):269-76
Concurrent lymph node metastases of medullary and papillary thyroid carcinoma in a case with RET oncogene germline mutation.
Papi G, Corrado S, Pomponi MG, Carapezzi C, Cesinaro A, LiVolsi VA
Endocrine pathology 2003 Fall;14(3):269-76
Endocrine pathology 2003 Fall;14(3):269-76
Classification of low-grade neuroendocrine tumors of midgut and unknown origin.
Van Eeden S, Quaedvlieg PF, Taal BG, Offerhaus GJ, Lamers CB, Van Velthuysen ML
Human pathology 2002 Nov;33(11):1126-32
Human pathology 2002 Nov;33(11):1126-32
Heparin-binding epidermal growth factor-like growth factor stimulates androgen-independent prostate tumor growth and antagonizes androgen receptor function.
Adam RM, Kim J, Lin J, Orsola A, Zhuang L, Rice DC, Freeman MR
Endocrinology 2002 Dec;143(12):4599-608
Endocrinology 2002 Dec;143(12):4599-608
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Chromogranin A using Chromogranin A Monoclonal Antibody (Product # MA5-13093) on PC12 Cells.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Chromogranin A (green) showing staining in the cytoplasm of SH-SY5Y cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Chromogranin A monoclonal antibody (Product # MA5-13096) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Chromogranin A (green) showing staining in the cytoplasm of U87-MG cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Chromogranin A monoclonal antibody (Product # MA5-13096) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Chromogranin A (green) showing staining in the cytoplasm of PC12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Chromogranin A monoclonal antibody (Product # MA5-13096) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human pancreatic tumor stained with Chromogranin A antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded rat pancreas stained with Chromogranin A antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of islet cells.
- Conjugate
- Biotin