NB120-15160

antibody from Novus Biologicals

Targeting: CHGA

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB120-15160

Provider

Novus Biologicals

Proper citation

Novus Cat#NB120-15160, RRID:AB_789299

Product name

Rabbit Polyclonal Chromogranin A Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Chromogranin A

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

1 ml

Concentration

1.0 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Formation of Human Neuroblastoma in Mouse-Human Neural Crest Chimeras.

Cohen MA, Zhang S, Sengupta S, Ma H, Bell GW, Horton B, Sharma B, George RE, Spranger S, Jaenisch R
Cell stem cell 2020 Apr 2;26(4):579-592.e6

---

Muc5ac null mice are predisposed to spontaneous gastric antro-pyloric hyperplasia and adenomas coupled with attenuated H. pylori-induced corpus mucous metaplasia.

Muthupalani S, Ge Z, Joy J, Feng Y, Dobey C, Cho HY, Langenbach R, Wang TC, Hagen SJ, Fox JG
Laboratory investigation; a journal of technical methods and pathology 2019 Dec;99(12):1887-1905

---

YAP inhibition enhances the differentiation of functional stem cell-derived insulin-producing β cells.

Rosado-Olivieri EA, Anderson K, Kenty JH, Melton DA
Nature communications 2019 Apr 1;10(1):1464

---

Characterization of Non-hormone Expressing Endocrine Cells in Fetal and Infant Human Pancreas.

Moin ASM, Montemurro C, Zeng K, Cory M, Nguyen M, Kulkarni S, Fritsch H, Meier JJ, Dhawan S, Rizza RA, Atkinson MA, Butler AE
Frontiers in endocrinology 2018;9:791

---

Paneth Cells Respond to Inflammation and Contribute to Tissue Regeneration by Acquiring Stem-like Features through SCF/c-Kit Signaling.

Schmitt M, Schewe M, Sacchetti A, Feijtel D, van de Geer WS, Teeuwssen M, Sleddens HF, Joosten R, van Royen ME, van de Werken HJG, van Es J, Clevers H, Fodde R
Cell reports 2018 Aug 28;24(9):2312-2328.e7

---

Pancreatic Nonhormone Expressing Endocrine Cells in Children With Type 1 Diabetes.

Md Moin AS, Cory M, Ong A, Choi J, Dhawan S, Butler PC, Butler AE
Journal of the Endocrine Society 2017 May 1;1(5):385-395

---

Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells.

Tüysüz N, van Bloois L, van den Brink S, Begthel H, Verstegen MM, Cruz LJ, Hui L, van der Laan LJ, de Jonge J, Vries R, Braakman E, Mastrobattista E, Cornelissen JJ, Clevers H, Ten Berge D
Nature communications 2017 Mar 6;8:14578

---

Increased Hormone-Negative Endocrine Cells in the Pancreas in Type 1 Diabetes.

Md Moin AS, Dhawan S, Shieh C, Butler PC, Cory M, Butler AE
The Journal of clinical endocrinology and metabolism 2016 Sep;101(9):3487-96

---

Increased Frequency of Hormone Negative and Polyhormonal Endocrine Cells in Lean Individuals With Type 2 Diabetes.

Md Moin AS, Dhawan S, Cory M, Butler PC, Rizza RA, Butler AE
The Journal of clinical endocrinology and metabolism 2016 Oct;101(10):3628-3636

---

Combined MYC Activation and Pten Loss Are Sufficient to Create Genomic Instability and Lethal Metastatic Prostate Cancer.

Hubbard GK, Mutton LN, Khalili M, McMullin RP, Hicks JL, Bianchi-Frias D, Horn LA, Kulac I, Moubarek MS, Nelson PS, Yegnasubramanian S, De Marzo AM, Bieberich CJ
Cancer research 2016 Jan 15;76(2):283-92

---

β-Cell Deficit in Obese Type 2 Diabetes, a Minor Role of β-Cell Dedifferentiation and Degranulation.

Butler AE, Dhawan S, Hoang J, Cory M, Zeng K, Fritsch H, Meier JJ, Rizza RA, Butler PC
The Journal of clinical endocrinology and metabolism 2016 Feb;101(2):523-32

---

p16(Ink4a)-induced senescence of pancreatic beta cells enhances insulin secretion.

Helman A, Klochendler A, Azazmeh N, Gabai Y, Horwitz E, Anzi S, Swisa A, Condiotti R, Granit RZ, Nevo Y, Fixler Y, Shreibman D, Zamir A, Tornovsky-Babeay S, Dai C, Glaser B, Powers AC, Shapiro AM, Magnuson MA, Dor Y, Ben-Porath I
Nature medicine 2016 Apr;22(4):412-20

---

Neuroendocrine differentiation correlates with hormone receptor expression and decreased survival in patients with invasive breast carcinoma.

Kwon SY, Bae YK, Gu MJ, Choi JE, Kang SH, Lee SJ, Kim A, Jung HR, Kang SH, Oh HK, Park JY
Histopathology 2014 Apr;64(5):647-59

---

Natural history and malignant risk factors of solid pseudopapillary tumors of the pancreas.

Park JK, Cho EJ, Ryu JK, Kim YT, Yoon YB
Postgraduate medicine 2013 Mar;125(2):92-9

---

Identification of adeno-associated viral vectors suitable for intestinal gene delivery and modulation of experimental colitis.

Polyak S, Mach A, Porvasnik S, Dixon L, Conlon T, Erger KE, Acosta A, Wright AJ, Campbell-Thompson M, Zolotukhin I, Wasserfall C, Mah C
American journal of physiology. Gastrointestinal and liver physiology 2012 Feb 1;302(3):G296-308

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry: Chromogranin A Antibody [NB120-15160] - IHC-P analysis fo human pancreas tissue showing specific staining of Chromogranin A in the Islets of Langerhans (dark brown staining).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Chromogranin A Antibody [NB120-15160] - IHC analysis of a formalin fixed paraffin embedded tissue section of mouse pancreas using Chromogranin A antibody at a dilution of 1:500. The signal was developed using HRP-conjugated anti-rabbit secondary and DAB reagent which followed counterstaining of the section with hematoxylin. The antibody generated a strong staining in Islets of Langerhans. Also, in the periphery of the cells, the pattern of staining was punctate representing the vesicles with Chromogranin A. Cells of lobules and acini showed a weak cytoplasmic and nuclear staining which is potentially the secreted pool of Chromogranin A.