16047-1-AP

antibody from Proteintech Group

Targeting: H4C5 H4/j, H4FJ, HIST1H4E

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

16047-1-AP

Provider

Proteintech Group

Proper citation

Proteintech Cat#16047-1-AP, RRID:AB_2118625

Product name

Histone H4 antibody

Antibody type

Polyclonal

Description

KD/KO validated Histone H4 antibody (Cat. #16047-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: ChIP, IF, IHC, IP, WB,ELISA.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

20ul, 150ul

References

Submitted references

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PRMT5 confers lipid metabolism reprogramming, tumour growth and metastasis depending on the SIRT7-mediated desuccinylation of PRMT5 K387 in tumours.

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The PRMT5-LSD1 axis confers Slug dual transcriptional activities and promotes breast cancer progression.

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Ribavirin inhibits colorectal cancer growth by downregulating PRMT5 expression and H3R8me2s and H4R3me2s accumulation.

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Arsenic influences spermatogenesis by disorganizing the elongation of spermatids in adult male mice.

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The Paf1 complex transcriptionally regulates the mitochondrial-anchored protein Atg32 leading to activation of mitophagy.

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Autophagy 2020 Aug;16(8):1366-1379

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PGN and LTA from Staphylococcus aureus Induced Inflammation and Decreased Lactation through Regulating DNA Methylation and Histone H3 Acetylation in Bovine Mammary Epithelial Cells.

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Toxins 2020 Apr 9;12(4)

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RAD6B Plays a Critical Role in Neuronal DNA Damage Response to Resist Neurodegeneration.

Guo Z, Tian Y, Guo Y, Li B, Liu X, Xie K, Song Y, Wang D
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Fluoride-induced unrestored arrest during haploid period of spermatogenesis via the regulation of DDX25 in rats.

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Epigenetic suppression of liver X receptor β in anterior cingulate cortex by HDAC5 drives CFA-induced chronic inflammatory pain.

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In the human sperm nucleus, nucleosomes form spatially restricted domains consistent with programmed nucleosome positioning.

Zhang MZ, Cao XM, Xu FQ, Liang XW, Fu LL, Li B, Liu WG, Li SG, Sun FZ, Huang XY, Huang WH
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Decreased synthesis of ribosomal proteins in tauopathy revealed by non-canonical amino acid labelling.

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MYST1/KAT8 contributes to tumor progression by activating EGFR signaling in glioblastoma cells.

Dong Z, Zou J, Li J, Pang Y, Liu Y, Deng C, Chen F, Cui H
Cancer medicine 2019 Dec;8(18):7793-7808

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NOS1 inhibits the interferon response of cancer cells by S-nitrosylation of HDAC2.

Xu P, Ye S, Li K, Huang M, Wang Q, Zeng S, Chen X, Gao W, Chen J, Zhang Q, Zhong Z, Lin Y, Rong Z, Xu Y, Hao B, Peng A, Ouyang M, Liu Q
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O-GlcNAc-Modification of NSL3 at Thr755 Site Maintains the Holoenzyme Activity of MOF/NSL Histone Acetyltransfease Complex.

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International journal of molecular sciences 2019 Dec 25;21(1)

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Function of RAD6B and RNF8 in spermatogenesis.

Guo Y, Song Y, Guo Z, Hu M, Liu B, Duan H, Wang L, Yuan T, Wang D
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The chromatin remodeling protein INO80 contributes to the removal of H2A.Z at the p53-binding site of the p21 gene in response to doxorubicin.

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The HIF/PHF8/AR axis promotes prostate cancer progression.

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Insights into Nucleosome Organization in Mouse Embryonic Stem Cells through Chemical Mapping.

Voong LN, Xi L, Sebeson AC, Xiong B, Wang JP, Wang X
Cell 2016 Dec 1;167(6):1555-1570.e15

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Western blot

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16047-1-AP(HIST1H4E antibody) at dilution of 1:500

Sample type

tissue

Immunohistochemistry

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

Immunohistochemical of paraffin-embedded human breast cancer using 16047-1-AP(HIST1H4E antibody) at dilution of 1:50 (under 10x lens)

Sample type

tissue

Supportive validation

Submitted by

Proteintech Group (provider)

Main image

Experimental details

The Histone H4 antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Histone H4. This antibody recognizes human,mouse,rat antigen. The Histone H4 antibody has been validated for the following applications: ELISA, IHC, WB analysis.

Appendix

16047-1-AP.pdf

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