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LearnPA5-93185
antibody from Invitrogen Antibodies
Targeting: SCYL1
GKLP, HT019, MGC78454, NKTL, NTKL, P105, TAPK, TEIF, TRAP
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-93185 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SCYL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: SASTRHRVLT SAFSRATRDP FAPSRVAGVL GFAATHNLYS MNDCAQKILP VLCGLTVDPE KSVRDQAFKA IRSFLSKLES VSEDPTQLEE VEKDVHAASS PGMGGAAASW AGWAVTGVSS LTSKLIRSHP TTAPTETNIP QRPTPEGVPA PAPTPVPATP TTSGHWETQE EDKDTAEDSS TADRWDDEDW GSLEQEAESV LAQQDDWSTG GQVSRASQVS NSDHKSSKSP ESDWSSWEAE GSWEQGWQEP SSQEPPPDGT RLASEYNWGG PESSDKGDPF A; Positive Samples: HeLa, Mouse pancreas; Cellular Location: Cytoplasm, Endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, Nucleus, centrosome, cis-Golgi network, cytoskeleton, microtubule organizing center
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.92 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts of various cell lines, using SCYL1 Polyclonal antibody (Product # PA5-93185) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of SCYL1 in extracts of various cell lines using SCYL1 Polyclonal Antibody (Product # PA5-93185) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of SCYL1 was performed in MCF-7 cells using SCYL1 Polyclonal Antibody (Product # PA5-93185).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SCYL1 in paraffin-embedded human liver damage using SCYL1 Polyclonal Antibody (Product # PA5-93185) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of SCYL1 in paraffin-embedded rat kidney using SCYL1 Polyclonal Antibody (Product # PA5-93185) at a dilution of 1:200.
