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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Chromatin Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-59201 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ER alpha/NR3A1 Antibody
- Antibody type
- Monoclonal
- Description
- ammonium sulphate purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 50 ug
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ER alpha/NR3A1 Antibody (C.15200009) [NBP2-59201] - Analysis was performed on 100 fmol ER alpha (ERa) and ER beta (ERb1) recombinant protein with the antibody directed against ER alpha at 7 ug/ mL. Figure shows the specificity of the antibody for the ER alpha isoform, whereas the ER beta isoform is not recognized.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: ER alpha/NR3A1 Antibody (C.15200009) [NBP2-59201] - ChIP using the antibody ChIP using the antibody against hER-alpha; from ChIP assays were performed using MCF7 cells, treated with the ER agonist estradiol for 3 hours prior to harvesting. The antibody directed against ER alpha and optimized primer sets for qPCR. Sheared chromatin from 3 million cells and 5 ug of antibody were used per ChIP experiment. Recovery (%: ChIP/input) and occupancy (x fold: +ve/-ve) are shown in figure. QPCR was performed with primers for the GREB1 promoter and for exon 2 of the myoglobin gene, used as a negative control. Figure shows the recovery (the relative amount of immunoprecipitated DNA compared to input DNA) and the occupancy (ratio +/- control target). These results demonstrate the occupancy of the GREB1 promoter by ERalpha.
