HPA000450

antibody from Atlas Antibodies

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA000450

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA000450, RRID:AB_1078754

Product name

Anti-ESR1

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human ESR1, Gene description: estrogen receptor 1, Alternative Gene Names: Era, ESR, NR3A1, Validated applications: WB, IHC, ChIP, Uniprot ID: P03372, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Single-cell analysis of breast cancer metastasis reveals epithelial-mesenchymal plasticity signatures associated with poor outcomes

Winkler J, Tan W, Diadhiou C, McGinnis C, Abbasi A, Hasnain S, Durney S, Atamaniuc E, Superville D, Awni L, Lee J, Hinrichs J, Wagner P, Singh N, Hein M, Borja M, Detweiler A, Liu S, Nanjaraj A, Sitarama V, Rugo H, Neff N, Gartner Z, Oliveira Pisco A, Goga A, Darmanis S, Werb Z
Journal of Clinical Investigation 2024;134(17)

---

CCL23 suppresses liver cancer progression through the CCR1/AKT/ESR1 feedback loop

Meng J, Wang L, Hou J, Yang X, Lin K, Nan H, Li M, Wu X, Chen X
Cancer Science 2021;112(8):3099-3110

---

Estrogen-Mediated Regulation of Mitochondrial Gene Expression

Lopez Sanchez M, Shearwood A, Chia T, Davies S, Rackham O, Filipovska A
Molecular Endocrinology 2015;29(1):14-27

---

Antibody performance in western blot applications is context‐dependent

Älgenäs C, Agaton C, Fagerberg L, Asplund A, Björling L, Björling E, Kampf C, Lundberg E, Nilsson P, Persson A, Wester K, Pontén F, Wernérus H, Uhlén M, Ottosson Takanen J, Hober S
Biotechnology Journal 2014;9(3):435-445

---

Antibody performance in western blot applications is context-dependent

Älgenäs C, Agaton C, Fagerberg L, Asplund A, Björling L, Björling E, Kampf C, Lundberg E, Nilsson P, Persson A, Wester K, Pontén F, Wernérus H, Uhlén M, Ottosson Takanen J, Hober S
Biotechnology Journal 2014 March;9(3):435-445

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and ESR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400046).

Sample type

Human

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human endometrium and cerebral cortex tissues using HPA000450 antibody. Corresponding ESR1 RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Show more

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

ChIP-Exo-Seq composite graph for Anti-ESR1 (HPA000450, Lot C74831) tested in MCF7 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.