PA5-96122

antibody from Invitrogen Antibodies

Targeting: H4C14 H4/n, H4F2, H4FN, HIST2H4, HIST2H4A

Western blot

ELISA

Antibody data

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Validation data

Product number: PA5-96122 - Provider product page
Provider: Invitrogen Antibodies
Product name: H4K20me1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Positive Samples: HeLa, H4 protein; Cellular Location: Chromosome, Nucleus Immunogen sequence: HRKVL
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 0.51 mg/mL
Storage: -20°C, Avoid Freeze/Thaw Cycles

H4C14 protein structure - PA5-96122 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H4K20me1 in paraffin-embedded human colon. Samples were incubated with H4K20me1 Polyclonal antibody (Product # PA5-96122) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H4K20me1 in paraffin-embedded mouse testis. Samples were incubated with H4K20me1 Polyclonal antibody (Product # PA5-96122) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H4K20me1 in paraffin-embedded rat testis. Samples were incubated with H4K20me1 Polyclonal antibody (Product # PA5-96122) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H4K20me1 in paraffin-embedded human colon. Samples were incubated with H4K20me1 Polyclonal antibody (Product # PA5-96122) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H4K20me1 in paraffin-embedded rat testis. Samples were incubated with H4K20me1 Polyclonal antibody (Product # PA5-96122) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of H4K20me1 in paraffin-embedded mouse testis. Samples were incubated with H4K20me1 Polyclonal antibody (Product # PA5-96122) using a dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.