Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB1958 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1958, RRID:AB_1577900
- Product name
- ERBB2 (phospho Y1248) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of ERBB2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Dietary (n-3) polyunsaturated fatty acids inhibit HER-2/neu-induced breast cancer in mice independently of the PPARgamma ligand rosiglitazone.
Effect of resveratrol on the development of spontaneous mammary tumors in HER-2/neu transgenic mice.
Identification and validation of novel ERBB2 (HER2, NEU) targets including genes involved in angiogenesis.
Yee LD, Young DC, Rosol TJ, Vanbuskirk AM, Clinton SK
The Journal of nutrition 2005 May;135(5):983-8
The Journal of nutrition 2005 May;135(5):983-8
Effect of resveratrol on the development of spontaneous mammary tumors in HER-2/neu transgenic mice.
Provinciali M, Re F, Donnini A, Orlando F, Bartozzi B, Di Stasio G, Smorlesi A
International journal of cancer 2005 May 20;115(1):36-45
International journal of cancer 2005 May 20;115(1):36-45
Identification and validation of novel ERBB2 (HER2, NEU) targets including genes involved in angiogenesis.
Beckers J, Herrmann F, Rieger S, Drobyshev AL, Horsch M, Hrabé de Angelis M, Seliger B
International journal of cancer 2005 Apr 20;114(4):590-7
International journal of cancer 2005 Apr 20;114(4):590-7
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) was used to detect phospho-ERBB2 in FG pancreatic carcinoma cell line lysate. FG pancreatic carcinoma cells treated with or without EGF (50 ug/mL) for 15 min ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) was used in 3% BSA. Data and protocol kindly provided by Dr. Weis of Cheresh Lab, UCSD.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of ERBB2 (phospho Y1248) polyclonal antibody (Cat # PAB1958) on nitrocellulose membrane. 50 ug of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
