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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Immunohistochemistry [1]
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- Product number
- A00010-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ErbB 2/ERBB2 Antibody Picoband™
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for Her2/ERBB2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Her2/ERBB2 information: Molecular Weight: 137910 MW; Subcellular Localization: Isoform 1: Cell membrane; Single-pass type I membrane protein. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1; Tissue Specificity: Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references Lapatinib-incorporated lipoprotein-like nanoparticles: preparation and a proposed breast cancer-targeting mechanism.
Incorporation of lapatinib into core-shell nanoparticles improves both the solubility and anti-glioma effects of the drug.
MiR199b suppresses expression of hypoxia-inducible factor 1α (HIF-1α) in prostate cancer cells.
In vitro study on human cytomegalovirus affecting early pregnancy villous EVT's invasion function.
Zhang L, Zhang S, Ruan SB, Zhang QY, He Q, Gao HL
Acta pharmacologica Sinica 2014 Jun;35(6):846-52
Acta pharmacologica Sinica 2014 Jun;35(6):846-52
Incorporation of lapatinib into core-shell nanoparticles improves both the solubility and anti-glioma effects of the drug.
Gao H, Wang Y, Chen C, Chen J, Wei Y, Cao S, Jiang X
International journal of pharmaceutics 2014 Jan 30;461(1-2):478-88
International journal of pharmaceutics 2014 Jan 30;461(1-2):478-88
MiR199b suppresses expression of hypoxia-inducible factor 1α (HIF-1α) in prostate cancer cells.
Shang W, Chen X, Nie L, Xu M, Chen N, Zeng H, Zhou Q
International journal of molecular sciences 2013 Apr 17;14(4):8422-36
International journal of molecular sciences 2013 Apr 17;14(4):8422-36
In vitro study on human cytomegalovirus affecting early pregnancy villous EVT's invasion function.
Tao L, Suhua C, Juanjuan C, Zongzhi Y, Juan X, Dandan Z
Virology journal 2011 Mar 11;8:114
Virology journal 2011 Mar 11;8:114
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Supportive validation
- Submitted by
- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of ErbB 2 using anti- ErbB 2 antibody (A00010-2). ErbB 2 was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- ErbB 2 Antibody (A00010-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image
